Humphrey D M, Hanahan D J, Pinckard R N
Lab Invest. 1982 Sep;47(3):227-34.
Intracutaneous injection of 5 to 50 pmoles of acetyl glyceryl ether phosphorylcholine (AGEPC) induced neutrophil (PMN) infiltrates in rabbit skin. PMN margination and plugging were present in dermal venules within 15 minutes after AGEPC injection, and extravascular PMN were most abundant after 3 hours. There was a sharp decline in PMN numbers when sites were examined 6 hours after AGEPC injection. PMN emigration appeared to occur predominantly from venules in the deep portion of the cutaneous venous plexus, and PMN accumulation was greatest in the deep dermis. Few if any PMN were present in lyso-glyceryl ether phosphorylcholine and human serum albumin sites at all time points studied. This demonstration that extravascularly introduced AGEPC induces PMN infiltrates in vivo provides evidence that this acetylated alkyl phosphoglyceride may be a significant mediator of acute allergic and inflammatory processes with a broader role than its previously described platelet-activating properties.
皮内注射5至50皮摩尔的乙酰甘油醚磷酸胆碱（AGEPC）可在兔皮肤中诱导中性粒细胞（PMN）浸润。注射AGEPC后15分钟内，真皮小静脉中出现PMN边缘化和阻塞，3小时后血管外PMN最为丰富。在注射AGEPC后6小时检查注射部位时，PMN数量急剧下降。PMN迁移似乎主要发生在皮肤静脉丛深部的小静脉，且PMN在真皮深部积聚最多。在所有研究的时间点，溶血甘油醚磷酸胆碱和人血清白蛋白注射部位几乎没有PMN。血管外引入的AGEPC在体内诱导PMN浸润的这一证明提供了证据，表明这种乙酰化烷基磷酸甘油酯可能是急性过敏和炎症过程的重要介质，其作用比先前描述的血小板活化特性更为广泛。
