甘油向β-羟基丙醛的细菌转化。
Bacterial conversion of glycerol to beta-hydroxypropionaldehyde.
作者信息
Vancauwenberge J E, Slininger P J, Bothast R J
机构信息
Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Illinois 61604.
出版信息
Appl Environ Microbiol. 1990 Feb;56(2):329-32. doi: 10.1128/aem.56.2.329-332.1990.
beta-hydroxypropionaldehyde (3-HPA) can be oxidized to acrylic acid, an industrially important chemical used in the manufacture of synthetic plastics and other polymers. Of 19 genera and 55 strains tested, 3 Klebsiella and 2 Enterobacter strains produced 3-HPA. The most efficient strain was Klebsiella pneumoniae NRRL B-4011. Under optimum conditions (28 degrees C; 40 g of semicarbazide hydrochloride per liter, 70 g of glycerol per liter; and pH 6.0), 3.1 g of B-4011 cells per liter accumulated 22 g of 3-HPA per liter at a specific rate of 0.83 g/g per h; however, 14.5 g of cells per liter accumulated 46 g of 3-HPA per liter at a specific rate of 0.41 g/g per h.
β-羟基丙醛（3-HPA）可被氧化为丙烯酸，丙烯酸是一种在合成塑料和其他聚合物制造中具有重要工业用途的化学品。在所测试的19个属和55个菌株中，3株克雷伯氏菌和2株肠杆菌菌株产生3-HPA。最有效的菌株是肺炎克雷伯氏菌NRRL B-4011。在最佳条件下（28℃；每升40克盐酸氨基脲、每升70克甘油；pH值6.0），每升3.1克B-4011细胞以每小时0.83克/克的比速率积累每升22克3-HPA；然而，每升14.5克细胞以每小时0.41克/克的比速率积累每升46克3-HPA。
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