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用于检测HIV抗体的western免疫印迹法及其解读。

The western immunoblot procedure for HIV antibodies and its interpretation.

作者信息

Dodd R Y, Fang C T

机构信息

Jerome H. Holland Laboratory, American Red Cross, Rockville, MD 20855.

出版信息

Arch Pathol Lab Med. 1990 Mar;114(3):240-5.

PMID:2407211
Abstract

The Western blot is frequently used to confirm human immunodeficiency virus type 1 (HIV-1) screening tests as it permits the identification of antibodies to individual component polypeptides of HIV-1. The procedure is described and different criteria for evaluation of HIV-1 Western blots are compared. The significance and management of indeterminate blot patterns are discussed.

摘要

蛋白质印迹法常用于确认1型人类免疫缺陷病毒（HIV-1）筛查试验，因为它能够识别针对HIV-1单个组成多肽的抗体。本文描述了该检测程序，并比较了评估HIV-1蛋白质印迹法的不同标准。同时讨论了不确定印迹模式的意义及处理方法。

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Arch Virol. 1993;130(3-4):413-28. doi: 10.1007/BF01309670.
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