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评估间接免疫荧光法在美国献血者血清中确认1型人类免疫缺陷病毒抗体的情况。

Evaluation of an indirect immunofluorescence assay for confirmation of human immunodeficiency virus type 1 antibody in U.S. blood donor sera.

作者信息

Sullivan M T, Mucke H, Kadey S D, Fang C T, Williams A E

机构信息

American Red Cross Holland Laboratory, Rockville, Maryland 20855.

出版信息

J Clin Microbiol. 1992 Sep;30(9):2509-10. doi: 10.1128/jcm.30.9.2509-2510.1992.

DOI:10.1128/jcm.30.9.2509-2510.1992
PMID:1401028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265538/
Abstract

An indirect immunofluorescence assay (IFA) was evaluated as a confirmatory test for antibody to human immunodeficiency virus type 1 in U.S. blood donor sera previously found to be repeatedly reactive by enzyme immunoassay. IFA results were 100% concordant with a licensed Western blot (immunoblot) for 53 negative and 49 positive samples. Four samples which exhibited antibody to viral proteins from more than one gene, yet were indeterminate by Western blot by the manufacturer's criteria, were also reactive by IFA, whereas 49 additional indeterminate samples were IFA negative.

摘要

间接免疫荧光测定法（IFA）被评估为一种确证试验，用于检测美国献血者血清中针对1型人类免疫缺陷病毒的抗体，这些血清先前经酶免疫测定法检测发现呈反复阳性反应。对于53份阴性样本和49份阳性样本，IFA结果与经许可的蛋白质印迹法（免疫印迹法）结果100%一致。有4份样本显示出针对来自多个基因的病毒蛋白的抗体，但根据制造商的标准经蛋白质印迹法检测结果为不确定，这些样本经IFA检测也呈阳性反应，而另外49份不确定样本经IFA检测为阴性。

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引用本文的文献

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本文引用的文献

1
Indirect immunofluorescence assay for antibodies to human immunodeficiency virus.人类免疫缺陷病毒抗体的间接免疫荧光测定法。
J Clin Microbiol. 1987 Feb;25(2):199-202. doi: 10.1128/jcm.25.2.199-202.1987.
2
Comparison of detection of antibody to the acquired immune deficiency syndrome virus by enzyme immunoassay, immunofluorescence, and Western blot methods.酶免疫测定、免疫荧光和蛋白质印迹法检测获得性免疫缺陷综合征病毒抗体的比较。
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Characterization of gp41 as the transmembrane protein coded by the HTLV-III/LAV envelope gene.
将gp41鉴定为由HTLV-III/LAV包膜基因编码的跨膜蛋白。
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Agreement study between two laboratories of immunofluorescence as a confirmatory test for human immunodeficiency virus type 1 antibody screening.两个实验室之间关于免疫荧光作为1型人类免疫缺陷病毒抗体筛查确证试验的一致性研究。
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The western immunoblot procedure for HIV antibodies and its interpretation.用于检测HIV抗体的western免疫印迹法及其解读。
Arch Pathol Lab Med. 1990 Mar;114(3):240-5.
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Frequency of indeterminate western blot tests in healthy adults at low risk for human immunodeficiency virus infection. The NIAID AIDS Vaccine Clinical Trails Network.低风险人类免疫缺陷病毒感染健康成年人中不确定的免疫印迹试验频率。美国国立过敏和传染病研究所艾滋病疫苗临床试验网络。
J Infect Dis. 1990 Dec;162(6):1379-82. doi: 10.1093/infdis/162.6.1379.