评估间接免疫荧光法在美国献血者血清中确认1型人类免疫缺陷病毒抗体的情况。
Evaluation of an indirect immunofluorescence assay for confirmation of human immunodeficiency virus type 1 antibody in U.S. blood donor sera.
作者信息
Sullivan M T, Mucke H, Kadey S D, Fang C T, Williams A E
机构信息
American Red Cross Holland Laboratory, Rockville, Maryland 20855.
出版信息
J Clin Microbiol. 1992 Sep;30(9):2509-10. doi: 10.1128/jcm.30.9.2509-2510.1992.
An indirect immunofluorescence assay (IFA) was evaluated as a confirmatory test for antibody to human immunodeficiency virus type 1 in U.S. blood donor sera previously found to be repeatedly reactive by enzyme immunoassay. IFA results were 100% concordant with a licensed Western blot (immunoblot) for 53 negative and 49 positive samples. Four samples which exhibited antibody to viral proteins from more than one gene, yet were indeterminate by Western blot by the manufacturer's criteria, were also reactive by IFA, whereas 49 additional indeterminate samples were IFA negative.
间接免疫荧光测定法（IFA）被评估为一种确证试验，用于检测美国献血者血清中针对1型人类免疫缺陷病毒的抗体，这些血清先前经酶免疫测定法检测发现呈反复阳性反应。对于53份阴性样本和49份阳性样本，IFA结果与经许可的蛋白质印迹法（免疫印迹法）结果100%一致。有4份样本显示出针对来自多个基因的病毒蛋白的抗体，但根据制造商的标准经蛋白质印迹法检测结果为不确定，这些样本经IFA检测也呈阳性反应，而另外49份不确定样本经IFA检测为阴性。
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