Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA.
1] Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [2] Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, USA [3] Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI, USA [4] Department of Urology, University of Michigan Medical School, Ann Arbor, MI, USA.
Mod Pathol. 2014 Apr;27(4):609-20. doi: 10.1038/modpathol.2013.169. Epub 2013 Sep 27.
PCA3 is a prostate-specific non-coding RNA, with utility as a urine-based early detection biomarker. Here, we report the evaluation of tissue PCA3 expression by RNA in situ hybridization in a cohort of 41 mapped prostatectomy specimens. We compared tissue PCA3 expression with tissue level ERG expression and matched pre-prostatectomy urine PCA3 and TMPRSS2-ERG levels. Across 136 slides containing 138 foci of prostate cancer, PCA3 was expressed in 55% of cancer foci and 71% of high-grade prostatic intraepithelial neoplasia foci. Overall, the specificity of tissue PCA3 was >90% for prostate cancer and high-grade prostatic intraepithelial neoplasia combined. Tissue PCA3 cancer expression was not significantly associated with urine PCA3 expression. PCA3 and ERG positivity in cancer foci was positively associated (P<0.01). We report the first comprehensive assessment of PCA3 expression in prostatectomy specimens, and find limited correlation between tissue PCA3 and matched urine in prostate cancer.
PCA3 是一种前列腺特异性非编码 RNA，可用作基于尿液的早期检测生物标志物。在这里，我们报告了对 41 个映射前列腺切除术标本中的组织 PCA3 表达进行 RNA 原位杂交评估的结果。我们将组织 PCA3 表达与组织水平的 ERG 表达以及匹配的前列腺前尿 PCA3 和 TMPRSS2-ERG 水平进行了比较。在包含 138 个前列腺癌病灶的 136 张幻灯片中，PCA3 在 55%的癌症病灶和 71%的高级别前列腺上皮内瘤变病灶中表达。总体而言，组织 PCA3 对前列腺癌和高级别前列腺上皮内瘤变的特异性>90%。组织 PCA3 癌症表达与尿 PCA3 表达无显著相关性。癌症病灶中 PCA3 和 ERG 的阳性表达呈正相关（P<0.01）。我们报告了前列腺切除术标本中 PCA3 表达的首次全面评估，发现组织 PCA3 与前列腺癌的匹配尿液之间相关性有限。
