Integrated Metabolomics Research Group, Seoul Center, Korea Basic Science Institute , Seoul 136-713, Republic of Korea.
J Agric Food Chem. 2013 Oct 30;61(43):10398-407. doi: 10.1021/jf4026103. Epub 2013 Oct 18.
A metabolomic analysis was performed to examine the postharvest processing of Astragalus membranaceus roots with a focus on the peeling procedure using (1)H NMR and UPLC-MS analyses. Principal component analysis (PCA) score plots from the (1)H NMR and UPLC-MS data showed clear separation between peeled and unpeeled Astragalus roots. Peeled roots exhibited significant losses of several primary metabolites, including acetate, alanine, arginine, caprate, fumarate, glutamate, histidine, N-acetylaspartate, malate, proline, sucrose, trigonelline, and valine. In contrast, the peeled roots contained higher levels of asparagine, aspartate, and xylose, which are xylem-related compounds, and formate, which is produced in response to wound stress incurred during postharvest processing. In addition, the levels of isoflavonoids and astragalosides were significantly reduced in peeled Astragalus root. These results demonstrate that metabolite profiling based on a combination of (1)H NMR and UPLC-MS analyses can be used to evaluate peeling procedures used in the postharvest processing of herbal medicines.
采用 1H NMR 和 UPLC-MS 分析方法对膜荚黄芪根的采后处理进行了代谢组学分析，重点研究了去皮过程。1H NMR 和 UPLC-MS 数据的主成分分析（PCA）得分图显示，去皮和未去皮的黄芪根之间有明显的分离。去皮根的几种主要代谢物显著损失，包括乙酸盐、丙氨酸、精氨酸、癸酸、富马酸、谷氨酸、组氨酸、N-乙酰天冬氨酸、苹果酸、脯氨酸、蔗糖、三叶草碱和缬氨酸。相比之下，去皮根含有更高水平的天冬酰胺、天冬氨酸和木糖，它们是木质部相关的化合物，以及甲酸盐，这是在采后加工过程中产生的伤口应激反应的产物。此外，去皮黄芪根中的异黄酮和黄芪苷的含量显著降低。这些结果表明，基于 1H NMR 和 UPLC-MS 分析相结合的代谢组学分析可用于评估中草药采后处理中使用的去皮程序。
