Liu Yang, Liu Jia, Wu Ke-Xin, Guo Xiao-Rui, Tang Zhong-Hua
Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China.
Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 15;1085:110-118. doi: 10.1016/j.jchromb.2018.03.044. Epub 2018 Apr 4.
Astragalus is one of the most popular Chinese herbal. Control of Astragalus quantity is most important, since that various varieties and ages largely affect bioactive metabolites and different pharmacological effects. Astragalus mongholicus and Astragalus membranaceus are both major sources of Astragalus according to the provisions in the Chinese Pharmacopoeia. Thus, a sensitive and rapid UPLC-MS/MS method for the simultaneous determination of l-Phenylalanine, Isoliquiritigenin, Liquiritigenin, Daidzein, Formononetin, Ononin, Calycosin, Calycosin-7-glucoside, Cycloastragenol, Astragaloside I, Astragaloside II, Astragaloside III and Astragaloside IV was established in this study. The detection was accomplished by MRM scanning in the positive ionization mode. Calibration curves offered linear ranges with r > 0.999. The method was successfully validated for the linearity, intra-day and inter day precisions, accuracy, recovery, matrix effect and stability. Then this method was successfully applied to detect the contents of 13 target flavonoids and triterpenoids metabolites in different organs and ages of A. mongholicus and A. membranaceus. Significant organs-, ages- and varieties- specificity of the 13 target metabolites were observed and discussed. The results provided basis and support for further exploration of the distribution of bioactive metabolites, namely flavonoids and triterpenoids, in different organs and ages of two Astragalus varieties. This method should be applicable to various Astragalus matrices for the quantitative analysis of the target flavonoids and triterpenoids.
黄芪是最常用的中草药之一。控制黄芪的质量至关重要，因为不同的品种和年份在很大程度上会影响生物活性代谢产物以及产生不同的药理作用。根据《中国药典》规定，蒙古黄芪和膜荚黄芪都是黄芪的主要来源。因此，本研究建立了一种灵敏、快速的超高效液相色谱-串联质谱法，用于同时测定L-苯丙氨酸、异甘草素、甘草素、大豆苷元、芒柄花素、芒柄花苷、毛蕊异黄酮、毛蕊异黄酮-7-葡萄糖苷、环黄芪醇、黄芪甲苷I、黄芪甲苷II、黄芪甲苷III和黄芪甲苷IV。检测采用正离子模式下的多反应监测扫描完成。校准曲线的线性范围为r＞0.999。该方法在线性、日内和日间精密度、准确度、回收率、基质效应和稳定性方面均得到成功验证。然后，该方法成功应用于检测蒙古黄芪和膜荚黄芪不同器官和年份中13种目标黄酮类和三萜类代谢产物的含量。观察并讨论了13种目标代谢产物显著的器官、年份和品种特异性。研究结果为进一步探究两种黄芪品种不同器官和年份中生物活性代谢产物（即黄酮类和三萜类）的分布提供了依据和支持。该方法应适用于各种黄芪基质中目标黄酮类和三萜类的定量分析。
