不育男性精液中与精液参数和精子 DNA 碎片化相关的大体积 DNA 加合物：一项横断面研究。
Bulky DNA adducts in human sperm associated with semen parameters and sperm DNA fragmentation in infertile men: a cross-sectional study.
机构信息
State Key Laboratory of Reproductive Medicine, Institute of Toxicology/Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
出版信息
Environ Health. 2013 Sep 30;12(1):82. doi: 10.1186/1476-069X-12-82.
BACKGROUND
DNA adducts are widely used marker of DNA damage induced by environmental pollutants. The present study was designed to explore whether sperm polycyclic aromatic hydrocarbon-DNA adducts were associated with sperm DNA integrity and semen quality.
METHODS
A total of 433 Han Chinese men were recruited from an infertility clinic. Immunofluorescence was applied to analyze sperm PAH-DNA adducts. Sperm DNA fragmentation was detected by terminal deoxynucleotidyl transferase (Tdt)-mediated dUTP nick end labelling (TUNEL) assay.
RESULTS
After adjustment for potential confounders using linear regression, sperm PAH-DNA adducts were negatively associated with sperm concentration, total sperm count, sperm motility, and curvilinear velocity (VCL). In addition, a positive relationship between sperm PAH-DNA adducts and sperm DNA fragmentation was found.
CONCLUSIONS
Our findings suggested an inverse association between sperm PAH-DNA adducts and semen quality, and provided the first epidemiologic evidence of an adverse effect of PAH-DNA adducts on sperm DNA integrity.
背景
DNA 加合物被广泛用作环境污染物诱导的 DNA 损伤标志物。本研究旨在探讨精子多环芳烃-DNA 加合物是否与精子 DNA 完整性和精液质量有关。
方法
从一家不孕不育诊所招募了 433 名汉族男性。应用免疫荧光法分析精子 PAH-DNA 加合物。采用末端脱氧核苷酸转移酶（Tdt）介导的 dUTP 缺口末端标记（TUNEL）法检测精子 DNA 碎片化。
结果
采用线性回归法调整潜在混杂因素后，精子 PAH-DNA 加合物与精子浓度、总精子数、精子活力和曲线速度（VCL）呈负相关。此外，还发现精子 PAH-DNA 加合物与精子 DNA 碎片化之间呈正相关关系。
结论
本研究结果表明，精子 PAH-DNA 加合物与精液质量呈负相关，并首次提供了 PAH-DNA 加合物对精子 DNA 完整性产生不良影响的流行病学证据。
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