School of Community and Environmental Health, College of Health Sciences, Old Dominion University, Norfolk, VA, USA.
J Hazard Mater. 2013 Jan 15;244-245:436-43. doi: 10.1016/j.jhazmat.2012.11.008. Epub 2012 Nov 26.
The objective of the cross-sectional study was to assess whether exposure to polycyclic aromatic hydrocarbons (PAHs) from coke oven emissions contributed to alteration of semen quality and sperm DNA integrity in nonsmoking workers. Nonsmoking coke oven workers from a steel plant in Taiwan served as the exposure groups (topside-oven workers for the high exposure group and side-oven workers for the low exposure group), and administrators and security personnel in the plant served as the control. An exposure assessment was conducted to determine both particulate and gaseous phase of PAH levels and urinary 1-hydroxypyrene (1-OHP) levels. Semen quality was analyzed according to WHO guidelines. DNA fragmentation and bulky DNA adducts were measured to assess sperm DNA integrity. There was no significant difference in sperm concentrations, vitality, and DNA fragmentation between the exposed group and the control. The high exposure group experienced significantly lower percentages of normal morphology as compared with the control (p=0.0001). Bulky DNA adducts were detected in the exposed group that were significant higher than the control (p=0.04). Exposure to PAHs from coke-oven emissions could contribute to increased levels of bulky DNA adducts in sperm.
本横断面研究旨在评估焦炉排放的多环芳烃（PAHs）暴露是否导致非吸烟工人的精液质量和精子 DNA 完整性发生改变。台湾一家钢铁厂的非吸烟焦炉工人作为暴露组（高温侧炉工人为高暴露组，低温侧炉工人为低暴露组），工厂的管理人员和保安人员作为对照组。进行了暴露评估，以确定 PAH 水平的颗粒相和气相以及尿 1-羟基芘（1-OHP）水平。根据世界卫生组织（WHO）的指南分析精液质量。测量精子 DNA 完整性的 DNA 片段化和大体积 DNA 加合物。与对照组相比，暴露组的精子浓度、活力和 DNA 片段化没有显著差异（p=0.0001）。与对照组相比，高暴露组正常形态的比例明显较低（p=0.0001）。暴露组检测到大体积 DNA 加合物显著高于对照组（p=0.04）。焦炉排放的多环芳烃暴露可能导致精子中大体积 DNA 加合物水平升高。
