• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

原位末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）与苯胺蓝法和流式细胞术吖啶橙法在测定精子DNA片段化和细胞核解聚状态指数方面的双盲比较。

A double-blinded comparison of in situ TUNEL and aniline blue versus flow cytometry acridine orange for the determination of sperm DNA fragmentation and nucleus decondensation state index.

作者信息

Hamidi Jamal, Frainais Christophe, Amar Edouard, Bailly Eric, Clément Patrice, Ménézo Yves

机构信息

Laboratoire Clément,17 avenue d'Eylau,75016 Paris and 8 avenue Henri Barbusse,93150 Le Blanc Mesnil,France.

Urology Department,American Hopsital of Paris,92200 Neuilly-sur-Seine,France.

出版信息

Zygote. 2015 Aug;23(4):556-62. doi: 10.1017/S0967199414000288. Epub 2014 Jul 3.

DOI:10.1017/S0967199414000288
PMID:24988915
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4501373/
Abstract

The impact of sperm DNA fragmentation on assisted reproductive technology (ART) successes, in terms of outcome, is now established. High levels of DNA strand breaks severely affect the probability of pregnancy. The importance of sperm nucleus condensation in early embryogenesis and, subsequently, on the quality of the conceptus is now emerging. In this article we have compared in situ analyses with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelling (TUNEL) (for DNA fragmentation) with aniline blue (AB) (for nucleus decondensation), versus flow cytometry (FC) after acridine orange staining, in a double-blinded analysis. In our hands, TUNEL and acridine orange give perfectly comparable results. For decondensation the results are also comparable, but the double-stranded green fluorescence obtained with acridine orange seems to slightly underestimate the decondensation status obtained with AB.

摘要

精子DNA片段化对辅助生殖技术（ART）成功结局的影响现已明确。高水平的DNA链断裂会严重影响怀孕几率。精子细胞核凝聚在早期胚胎发育以及随后对胚胎质量的重要性正逐渐显现。在本文中，我们在双盲分析中，将原位分析（采用末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记法（TUNEL）检测DNA片段化，苯胺蓝（AB）检测细胞核去凝聚）与吖啶橙染色后的流式细胞术（FC）进行了比较。在我们的研究中，TUNEL和吖啶橙给出的结果完全可比。对于去凝聚情况，结果同样具有可比性，但吖啶橙获得的双链绿色荧光似乎略微低估了AB所检测到的去凝聚状态。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/a969fac58129/S0967199414000288_fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/be9a46dcb543/S0967199414000288_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/f0c3c676c719/S0967199414000288_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/5705930509c8/S0967199414000288_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/a969fac58129/S0967199414000288_fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/be9a46dcb543/S0967199414000288_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/f0c3c676c719/S0967199414000288_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/5705930509c8/S0967199414000288_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b03/4501373/a969fac58129/S0967199414000288_fig4.jpg

相似文献

1
A double-blinded comparison of in situ TUNEL and aniline blue versus flow cytometry acridine orange for the determination of sperm DNA fragmentation and nucleus decondensation state index.原位末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）与苯胺蓝法和流式细胞术吖啶橙法在测定精子DNA片段化和细胞核解聚状态指数方面的双盲比较。
Zygote. 2015 Aug;23(4):556-62. doi: 10.1017/S0967199414000288. Epub 2014 Jul 3.
2
Clinical aspects of sperm DNA fragmentation detection and male infertility.精子DNA片段化检测与男性不育的临床方面
Theriogenology. 2006 Mar 15;65(5):979-91. doi: 10.1016/j.theriogenology.2005.09.011. Epub 2005 Oct 19.
3
Human sperm DNA fragmentation: correlation of TUNEL results as assessed by flow cytometry and optical microscopy.人类精子DNA片段化：流式细胞术和光学显微镜评估的TUNEL结果的相关性
Cytometry A. 2007 Dec;71(12):1011-8. doi: 10.1002/cyto.a.20484.
4
The Sperm Chromatin Structure Assay (SCSA(®)) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility.精子染色质结构分析（SCSA(®)）及其他用于评估与生育力相关的精子核DNA完整性的精子DNA碎片化检测。
Anim Reprod Sci. 2016 Jun;169:56-75. doi: 10.1016/j.anireprosci.2016.01.017. Epub 2016 Feb 17.
5
DNA double strand breaks in human spermatozoa can be predictive for assisted reproductive outcome.人类精子中的DNA双链断裂可预测辅助生殖结果。
Reprod Biomed Online. 2015 Jul;31(1):100-7. doi: 10.1016/j.rbmo.2015.03.009. Epub 2015 Apr 8.
6
Influence of deoxyribonucleic acid damage on fertilization and pregnancy.脱氧核糖核酸损伤对受精和妊娠的影响。
Fertil Steril. 2004 Apr;81(4):965-72. doi: 10.1016/j.fertnstert.2003.09.044.
7
High-throughput analysis of TUNEL-stained sperm using image cytometry.应用图像细胞术对 TUNEL 染色精子进行高通量分析。
Cytometry A. 2017 Sep;91(9):854-858. doi: 10.1002/cyto.a.23164. Epub 2017 Jul 5.
8
Paternal age and sperm DNA decay: discrepancy between chromomycin and aniline blue staining.
Reprod Biomed Online. 2009 Aug;19(2):264-9. doi: 10.1016/s1472-6483(10)60083-1.
9
Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry.使用两种不同方法评估精子 DNA 碎片化：荧光显微镜下的 TUNEL 法和流式细胞术。
Medicina (Kaunas). 2023 Jul 15;59(7):1313. doi: 10.3390/medicina59071313.
10
Comparative analysis of three sperm DNA damage assays and sperm nuclear protein content in couples undergoing assisted reproduction treatment.接受辅助生殖治疗的夫妇中三种精子DNA损伤检测方法与精子核蛋白含量的比较分析。
Hum Reprod. 2014 May;29(5):904-17. doi: 10.1093/humrep/deu040. Epub 2014 Mar 10.

引用本文的文献

1
Predicting Boar Sperm Survival during Liquid Storage Using Vibrational Spectroscopic Techniques.使用振动光谱技术预测公猪精子在液体保存期间的存活情况。
Biology (Basel). 2024 Sep 26;13(10):763. doi: 10.3390/biology13100763.
2
Sperm selection with hyaluronic acid improved live birth outcomes among older couples and was connected to sperm DNA quality, potentially affecting all treatment outcomes.使用透明质酸进行精子选择可改善高龄夫妇的活产结局，并与精子 DNA 质量有关，可能影响所有治疗结局。
Hum Reprod. 2022 May 30;37(6):1106-1125. doi: 10.1093/humrep/deac058.
3
Mutations and Teratozoospermia: Genetic Effects on Sperm Head Morphology and DNA Integrity.

本文引用的文献

1
The importance of the one carbon cycle nutritional support in human male fertility: a preliminary clinical report.一碳循环营养支持对男性生育能力的重要性：初步临床报告
Reprod Biol Endocrinol. 2014 Jul 29;12:71. doi: 10.1186/1477-7827-12-71.
2
Assessment of chromatin maturity in human spermatozoa: useful aniline blue assay for routine diagnosis of male infertility.人类精子中染色质成熟度的评估：用于男性不育症常规诊断的实用苯胺蓝检测法。
Adv Urol. 2013;2013:578631. doi: 10.1155/2013/578631. Epub 2013 Oct 3.
3
Bulky DNA adducts in human sperm associated with semen parameters and sperm DNA fragmentation in infertile men: a cross-sectional study.
突变与畸形精子症：对精子头部形态和DNA完整性的遗传影响
J Clin Med. 2019 Aug 23;8(9):1297. doi: 10.3390/jcm8091297.
4
Evaluation of Sperm Chromatin Integrity Using Aniline Blue and Toluidine Blue Staining in Infertile and Normozoospermic Men.使用苯胺蓝和甲苯胺蓝染色评估不育和正常精子男性的精子染色质完整性
J Reprod Infertil. 2019 Apr-Jun;20(2):95-101.
5
Evaluation of sperm DNA structure, fragmentation and decondensation: an essential tool in the assessment of male infertility.精子DNA结构、片段化及解聚的评估：男性不育评估中的一项重要工具。
Transl Androl Urol. 2017 Sep;6(Suppl 4):S553-S556. doi: 10.21037/tau.2017.03.11.
6
Evaluation of sperm chromatin structure and DNA strand breaks is an important part of clinical male fertility assessment.评估精子染色质结构和DNA链断裂是临床男性生育力评估的重要组成部分。
Transl Androl Urol. 2017 Sep;6(Suppl 4):S495-S500. doi: 10.21037/tau.2017.07.20.
7
Association between the MTHFR-C677T isoform and structure of sperm DNA.亚甲基四氢叶酸还原酶 C677T 基因型与精子 DNA 结构的关系。
J Assist Reprod Genet. 2017 Oct;34(10):1283-1288. doi: 10.1007/s10815-017-1015-2. Epub 2017 Aug 25.
不育男性精液中与精液参数和精子 DNA 碎片化相关的大体积 DNA 加合物：一项横断面研究。
Environ Health. 2013 Sep 30;12(1):82. doi: 10.1186/1476-069X-12-82.
4
Effect of antioxidants on sperm genetic damage.抗氧化剂对精子遗传损伤的影响。
Adv Exp Med Biol. 2014;791:173-89. doi: 10.1007/978-1-4614-7783-9_11.
5
The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment.精子 DNA 损伤对辅助受孕的影响及其他方面：诊断和治疗的新进展。
Reprod Biomed Online. 2013 Oct;27(4):325-37. doi: 10.1016/j.rbmo.2013.06.014. Epub 2013 Jul 11.
6
The utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis.在常规精液分析中使用甲苯胺蓝和苯胺蓝染色进行精子DNA损伤检测的效用。
Clin Exp Reprod Med. 2013 Mar;40(1):23-8. doi: 10.5653/cerm.2013.40.1.23. Epub 2013 Mar 31.
7
New insights into human pre-implantation metabolism in vivo and in vitro.体内和体外人类植入前代谢的新见解。
J Assist Reprod Genet. 2013 Mar;30(3):293-303. doi: 10.1007/s10815-013-9953-9. Epub 2013 Feb 21.
8
Reproductive technologies and the risk of birth defects.生殖技术与出生缺陷的风险。
N Engl J Med. 2012 May 10;366(19):1803-13. doi: 10.1056/NEJMoa1008095. Epub 2012 May 5.
9
Sperm nucleus decondensation, hyaluronic acid (HA) binding and oocyte activation capacity: different markers of sperm immaturity? Case reports.精子核去浓缩、透明质酸（HA）结合及卵母细胞激活能力：精子不成熟的不同标志物？病例报告。
J Assist Reprod Genet. 2012 Apr;29(4):353-5. doi: 10.1007/s10815-012-9710-5. Epub 2012 Jan 18.
10
Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success.精子 DNA 损伤的碱性彗星试验检测作为男性不育和体外受精成功的独立预测因子。
Fertil Steril. 2011 Feb;95(2):652-7. doi: 10.1016/j.fertnstert.2010.08.019. Epub 2010 Sep 22.