Department of Biochemistry, Radboud University Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands.
Nanomedicine (Lond). 2013 Oct;8(10):1657-68. doi: 10.2217/nnm.13.149.
Microparticles (MPs) are small plasma membrane-derived vesicles that can expose molecules originating from their parental cells. As vectors of biological information they are likely to play an active role in both homeostasis and pathogenesis, making them promising biomarkers and nanomedicine tools. Therefore, there is an urgent need for standardization of MP isolation and analysis protocols to propel our understanding of MP biology to the next level. Based on current methodology and recent insights, this review proposes an optimized protocol for the isolation and biochemical characterization of MPs.
微粒（MPs）是小的质膜衍生小泡，可暴露源自其亲本细胞的分子。作为生物信息的载体，它们可能在体内平衡和发病机制中发挥积极作用，使它们成为有前途的生物标志物和纳米医学工具。因此，迫切需要标准化 MP 分离和分析方案，以将我们对 MP 生物学的理解提升到一个新的水平。基于当前的方法和最近的研究进展，本文提出了一种优化的 MP 分离和生化特性分析方案。
