Winkler Cornelia, Schäfer Lina, Felthaus Oliver, Allerdings Juri, Hahnel Sebastian, Behr Michael, Bürgers Ralf
Department of Prosthetic Dentistry, Regensburg University Medical Center, Regensburg, Germany.
Acta Odontol Scand. 2014 May;72(4):241-50. doi: 10.3109/00016357.2013.828320. Epub 2013 Sep 30.
Bacterial adhesion on and cytotoxicity of eight luting agents used for implant-supported restorations were investigated.
Surface roughness (Ra), surface free energy (SFE) values and three-dimensional images by atomic-force microscopy of circular specimens were determined. Bacterial suspensions of Streptococcus sanguinis and Streptococcus epidermidis were incubated at 37°C for 2 h. Adhering bacteria were examined with fluorescence dye CytoX-Violet, stained with 4',6-diamidino-2-phenylindole (DAPI) and visualized by fluorescence-microscopy. Cytotoxicity-testing was done with WST-1-tests (water soluble tetrazolium). No significant differences, neither with regard to Ra nor regarding SFE were determined.
Adherence of S. sanguinis was less on titanium, TempBondNE and TempBond. TempBond, TempBondNE, RelyX Unicem and Implantlink Semi Classic presented low amounts of S. epidermidis. WST-testing showed high cytotoxic potential of Harvard, Aqualox, TempBondNE and TempBond. No combination of low adherent bacteria with low cytotoxicity was found.
From a biological in-vitro perspective, none of the cements may be recommended for implant-supported restorations.
研究用于种植体支持修复体的八种粘结剂的细菌粘附情况及细胞毒性。
测定圆形样本的表面粗糙度（Ra）、表面自由能（SFE）值以及通过原子力显微镜获得的三维图像。将血链球菌和表皮葡萄球菌的细菌悬液在37°C孵育2小时。用荧光染料CytoX - Violet检测粘附细菌，用4',6 - 二脒基 - 2 - 苯基吲哚（DAPI）染色，并通过荧光显微镜观察。用WST - 1试验（水溶性四氮唑）进行细胞毒性测试。在Ra和SFE方面均未确定显著差异。
血链球菌在钛、TempBondNE和TempBond上的粘附较少。TempBond、TempBondNE、RelyX Unicem和Implantlink Semi Classic上的表皮葡萄球菌数量较少。WST测试显示Harvard、Aqualox、TempBondNE和TempBond具有较高的细胞毒性潜力。未发现低粘附细菌与低细胞毒性的组合。
从生物体外角度来看，没有一种粘结剂可推荐用于种植体支持的修复体。
