Pediatric Infectious Diseases, University Children's Hospital Mannheim of Heidelberg University, Germany.
Nutr Res. 2013 Oct;33(10):831-8. doi: 10.1016/j.nutres.2013.07.009. Epub 2013 Aug 13.
Human milk oligosaccharides help to prevent infectious diseases in breastfed infants. Larger scale testing, particularly in animal models and human clinical studies, is still limited due to shortened availability of more complex oligosaccharides. The purpose of this study was to evaluate 2'-fucosyllactose (2'-FL) and 3-fucosyllactose (3-FL) synthesized by whole-cell biocatalysis for their biological activity in vitro. Therefore, we have tested these oligosaccharides for their inhibitory potential of pathogen adhesion in two different human epithelial cell lines. 2'-FL could inhibit adhesion of Campylobacter jejuni, enteropathogenic Escherichia coli, Salmonella enterica serovar fyris, and Pseudomonas aeruginosa to the intestinal human cell line Caco-2 (reduction of 26%, 18%, 12%, and 17%, respectively), as could be shown for 3-FL (enteropathogenic E coli 29%, P aeruginosa 26%). Furthermore, adherence of P aeruginosa to the human respiratory epithelial cell line A549 was significantly inhibited by 2'-FL and 3-FL (reduction of 24% and 23%, respectively). These results confirm the biological and functional activity of biotechnologically synthesized human milk oligosaccharides. Mass-tailored human milk oligosaccharides could be used in the future to supplement infant formula ingredients or as preventatives to reduce the impact of infectious diseases.
人乳寡糖有助于预防母乳喂养婴儿的感染性疾病。由于更复杂的寡糖的供应时间缩短，更大规模的测试，特别是在动物模型和人体临床研究中，仍然受到限制。本研究的目的是评估全细胞生物催化合成的 2'-岩藻糖基乳糖（2'-FL）和 3-岩藻糖基乳糖（3-FL）在体外的生物学活性。因此，我们测试了这些寡糖抑制两种不同人上皮细胞系中病原体黏附的潜力。2'-FL 可以抑制弯曲杆菌、肠致病性大肠杆菌、肠炎沙门氏菌 fyris 和铜绿假单胞菌与肠道人细胞系 Caco-2 的黏附（分别减少 26%、18%、12%和 17%），3-FL 也可以抑制肠致病性大肠杆菌（减少 29%）、铜绿假单胞菌（减少 26%）的黏附。此外，2'-FL 和 3-FL 显著抑制铜绿假单胞菌对人呼吸道上皮细胞系 A549 的黏附（分别减少 24%和 23%）。这些结果证实了生物技术合成的人乳寡糖的生物学和功能活性。未来可以使用大规模定制的人乳寡糖来补充婴儿配方成分或作为预防措施，以降低传染病的影响。
