Canadian Food Inspection Agency, Animal Health Microbacteriology Laboratory (Germplasm), Ottawa, Ontario, Canada.
Theriogenology. 2013 Dec;80(9):1104-8. doi: 10.1016/j.theriogenology.2013.08.012. Epub 2013 Sep 26.
This study deals with the potential for the introduction of infectious agents through the use of animal-derived products. The efficacy of a recombinant bovine trypsin (RBTr) as a replacement for porcine pancreatic trypsin and a disinfectant for bovine herpesvirus-1 (BHV-1)-infected embryos was investigated according to the sanitary guidelines of the International Embryo Transfer Society. Treatment of in vivo and in vitro fertilized embryos contaminated with BHV-1 (10(5) TCID50/mL) in the presence of RBTr (525 U/mL) for 120 s, effectively removed the infectious virus compared with untreated and washed embryos (P < 0.05). Transfer of in vivo fertilized and disinfected embryos to BHV-1 seronegative recipients (n = 24) resulted in 14 pregnancies and 11 calves born free of BHV-1. In contrast, transfer of unwashed or undisinfected embryos to four recipients resulted in seroconversion and no pregnancies at term. It was concluded that the use of RBTr could be considered as an alternative method of rendering embryos free of BHV-1 and thus reduce the potential risk of disease transmission to embryo recipients and offspring.
本研究探讨了通过使用动物源性产品引入感染性因子的可能性。根据国际胚胎移植协会的卫生指南，研究了重组牛胰蛋白酶（RBTr）作为猪胰蛋白酶替代物和用于牛疱疹病毒 1（BHV-1）感染胚胎的消毒剂的功效。在存在 RBTr（525 U/mL）的情况下，处理体内和体外受精胚胎中被 BHV-1 污染（10(5)TCID50/mL），与未经处理和洗涤的胚胎相比，有效去除了传染性病毒（P<0.05）。将体内受精和消毒的胚胎移植到 BHV-1 血清阴性受体（n=24）中，导致 14 例怀孕和 11 头无 BHV-1 的小牛出生。相比之下，将未经洗涤或未经消毒的胚胎移植到四个受体中，导致血清转化且没有足月怀孕。研究结论认为，RBTr 的使用可以被视为使胚胎无 BHV-1 的替代方法，从而降低向胚胎受体和后代传播疾病的潜在风险。
