Department of Plant Pathology, Washington State University, Pullman, WA 99164-106, United States; Agriculture and Agri-Food Canada, P.O. Box 3000, Lethbridge, Alberta T1J 4B1, Canada.
Virus Res. 2013 Dec 26;178(2):306-13. doi: 10.1016/j.virusres.2013.09.026. Epub 2013 Sep 25.
Rubus yellow net virus (RYNV) was cloned and sequenced from a red raspberry (Rubus idaeus L.) plant exhibiting symptoms of mosaic and mottling in the leaves. Its genomic sequence indicates that it is a distinct member of the genus Badnavirus, with 7932bp and seven ORFs, the first three corresponding in size and location to the ORFs found in the type member Commelina yellow mottle virus. Bioinformatic analysis of the genomic sequence detected several features including nucleic acid binding motifs, multiple zinc finger-like sequences and domains associated with cellular signaling. Subsequent sequencing of the small RNAs (sRNAs) from RYNV-infected R. idaeus leaf tissue was used to determine any RYNV sequences targeted by RNA silencing and identified abundant virus-derived small RNAs (vsRNAs). The majority of the vsRNAs were 22-nt in length. We observed a highly uneven genome-wide distribution of vsRNAs with strong clustering to small defined regions distributed over both strands of the RYNV genome. Together, our data show that sequences of the aphid-transmitted pararetrovirus RYNV are targeted in red raspberry by the interfering RNA pathway, a predominant antiviral defense mechanism in plants.
覆盆子黄网病毒（RYNV）是从表现叶片花叶和斑驳症状的红树莓（Rubus idaeus L.）植物中克隆和测序的。其基因组序列表明，它是 Badnavirus 属的一个独特成员，具有 7932bp 和七个 ORF，前三个 ORF 的大小和位置与典型成员鸭跖草黄斑驳病毒的 ORF 相对应。对基因组序列的生物信息学分析检测到了几个特征，包括核酸结合基序、多个锌指样序列和与细胞信号相关的结构域。随后对 RYNV 感染的红树莓叶片组织中的小 RNA（sRNA）进行测序，以确定任何被 RNA 沉默靶向的 RYNV 序列，并鉴定出丰富的病毒衍生小 RNA（vsRNA）。大多数 vsRNA 的长度为 22nt。我们观察到 vsRNA 在整个基因组上的分布极不均匀，强烈聚集在 RYNV 基因组两条链上的小定义区域。综上所述，我们的数据表明，蚜虫传播的副逆转录病毒 RYNV 序列在红树莓中被干扰 RNA 途径靶向，这是植物中主要的抗病毒防御机制。
