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酿酒酵母中非环状多元醇的转运

Transport of acyclic polyols in Saccharomyces cerevisiae.

作者信息

Canh D S, Horák J, Kotyk A, Ríhová L

出版信息

Folia Microbiol (Praha). 1975;20(4):320-5. doi: 10.1007/BF02878113.

DOI:10.1007/BF02878113
PMID:240765
Abstract

Acyclic polyols (erythritol, xylitol, ribitol, D-arabinitol, mannitol, sorbitol and galactitol) are not metabolized by Saccharomyces cerevisiae. They are taken up by a fast non-active process, reaching 40-70% distribution referred to total cell water. The uptake is insensitive to temperature, pH (between 4 and 8), 2,4-dinitrophenol and uranyl ions. Its initial rate rises linearly with concentration from 10(-5)M to 1M. The process resembles simple diffusion through large pores or the trapping of the whole solution on the surface. Protoplasts behave like whole cells in this respect. Only erythritol shows a second type of uptake which is inhibitor-insensitive but temperature-dependent.

摘要

无环多元醇(赤藓糖醇、木糖醇、核糖醇、D-阿拉伯糖醇、甘露醇、山梨醇和半乳糖醇)不能被酿酒酵母代谢。它们通过快速非活性过程被吸收,相对于总细胞水达到40 - 70%的分布。这种吸收对温度、pH值(4至8之间)、2,4 - 二硝基苯酚和铀离子不敏感。其初始速率随浓度从10⁻⁵M到1M呈线性上升。该过程类似于通过大孔的简单扩散或整个溶液在表面的截留。在这方面,原生质体的行为与完整细胞相似。只有赤藓糖醇表现出第二种吸收类型,这种吸收对抑制剂不敏感,但依赖于温度。

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本文引用的文献

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Membrane changes in yeast cells caused by sulfhydryl reagents and accompanied by a selective release of sugar.巯基试剂引起的酵母细胞的膜变化,并伴有糖的选择性释放。
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Properties of the sugar carrier in baker's yeast. II. Specificity of transport.面包酵母中糖载体的特性。II. 运输特异性。
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