Roboz J, Kappatos D C, Greaves J, Holland J F
Clin Chem. 1984 Oct;30(10):1611-5.
We used gas chromatography-chemical ionization mass spectrometry with selected ion monitoring of the (M-59)+ ions to determine polyols (as their peracetyl derivatives) in serum. The internal standard was 2-deoxygalactitol. Mean (and SD) polyol concentrations (mg/L) in 33 normal sera were: erythritol 0.45 (0.14), threitol 0.20 (0.06), adonitol 0.06 (0.02), arabinitol 0.37 (0.12), xylitol 0.05 (0.02), mannitol 0.41 (0.45), galactitol 0.15 (0.11), sorbitol 0.16 (0.11). Arabinitol determined in the same 33 normal sera by trimethylsilylation was 0.39 (0.13) mg/L. In 32 samples from cancer patients with normal creatinine, all polyol concentrations were in the normal range except for seven increased erythritol and one increased threitol concentrations. In all six patients with renal dysfunction we found increased erythritol and arabinitol, and increased threitol and mannitol in four of the six. Initial results showed that only arabinitol concentrations increased in invasive candidiasis.
我们采用气相色谱 - 化学电离质谱法，通过对（M - 59）⁺离子进行选择离子监测，来测定血清中的多元醇（以其全乙酰化衍生物形式）。内标为2 - 脱氧半乳糖醇。33份正常血清中多元醇的平均（及标准差）浓度（mg/L）分别为：赤藓醇0.45（0.14），苏糖醇0.20（0.06），阿东糖醇0.06（0.02），阿拉伯糖醇0.37（0.12），木糖醇0.05（0.02），甘露醇0.41（0.45），半乳糖醇0.15（0.11），山梨醇0.16（0.11）。通过三甲基硅烷化法测定的这33份正常血清中的阿拉伯糖醇浓度为0.39（0.13）mg/L。在32份肌酐正常的癌症患者样本中，除7份样本的赤藓醇浓度升高和1份样本的苏糖醇浓度升高外，所有多元醇浓度均在正常范围内。在所有6例肾功能不全患者中，我们发现赤藓醇和阿拉伯糖醇浓度升高，6例中有4例苏糖醇和甘露醇浓度升高。初步结果显示，侵袭性念珠菌病患者仅阿拉伯糖醇浓度升高。
