Kumaratilake L M, Ferrante A, Rzepczyk C M
Department of Immunology, Adelaide Children's Hospital, South Australia.
Infect Immun. 1990 Mar;58(3):788-93. doi: 10.1128/iai.58.3.788-793.1990.
We developed a radiometric assay by which the antiplasmodial effects of phagocytic cells can be quantitated. This assay was used to examine the effects of recombinant human tumor necrosis factor alpha (TNF-alpha) on the killing of Plasmodium falciparum by human neutrophils. Data presented demonstrated that neutrophils engulf and destroy P. falciparum, but substantial killing of parasites required the presence of either heat-labile or heat-stable opsonins. While recombinant TNF-alpha at concentrations of 5 to 50,000 U/ml showed no direct effects on the parasite, this cytokine augmented the antimalarial activity of neutrophils at doses of 20 to 250 U/10(6) neutrophils. The results suggest that TNF-alpha is an important component of the immune phagocytic effector mechanisms which are involved in destruction of the malarial parasite.
我们开发了一种放射测定法，通过该方法可以定量吞噬细胞的抗疟原虫作用。该测定法用于检测重组人肿瘤坏死因子α（TNF-α）对人中性粒细胞杀伤恶性疟原虫的影响。所呈现的数据表明，中性粒细胞吞噬并破坏恶性疟原虫，但大量杀伤寄生虫需要存在热不稳定或热稳定的调理素。虽然浓度为5至50,000 U/ml的重组TNF-α对寄生虫没有直接影响，但这种细胞因子在20至250 U/10(6)中性粒细胞的剂量下增强了中性粒细胞的抗疟活性。结果表明，TNF-α是参与疟原虫破坏的免疫吞噬效应机制的重要组成部分。
