Kumaratilake L M, Ferrante A, Jaeger T, Rzepczyk C
Department of Immunology, Women's and Children's Hospital, Adelaide, Australia.
Parasite Immunol. 1996 Mar;18(3):115-23. doi: 10.1046/j.1365-3024.1996.d01-64.x.
Granulocyte macrophage-colony stimulation factor (GM-CSF), which is a haematopoietic cytokine generated by activated T lymphocytes and macrophages during infection, was investigated for its effects on human neutrophil-mediated killing of asexual blood forms of Plasmodium falciparum. Pretreatment of neutrophils with human recombinant-GM-CSF markedly increased the parasite killing (measured by a radiometric assay), in the presence of normal serum (containing complement), immune serum (IS), purified IgG (from IS) or heat inactivated IS. GM-CSF pretreatment also enhanced phagocytosis of the parasite by neutrophils and the expression of CR3, Fc gamma RII and Fc gamma RIII receptors. Treatment of neutrophils with a combination of GM-CSF and TNF resulted in a synergistic increase in phagocytosis and killing of the parasite. The findings suggest that GM-CSF is likely to form part of the cytokine network responsible for regulating the antiparasitic activity of the neutrophil in malaria.
粒细胞巨噬细胞集落刺激因子（GM-CSF）是一种在感染期间由活化的T淋巴细胞和巨噬细胞产生的造血细胞因子，本研究探讨了其对人类中性粒细胞介导的恶性疟原虫无性血液期虫体杀伤作用的影响。在正常血清（含补体）、免疫血清（IS）、纯化的IgG（来自IS）或热灭活的IS存在的情况下，用人重组GM-CSF对中性粒细胞进行预处理，可显著增强寄生虫杀伤作用（通过放射性测定法测量）。GM-CSF预处理还增强了中性粒细胞对寄生虫的吞噬作用以及CR3、FcγRII和FcγRIII受体的表达。用GM-CSF和TNF联合处理中性粒细胞可导致吞噬作用和寄生虫杀伤作用协同增强。这些发现表明，GM-CSF可能是负责调节疟疾中中性粒细胞抗寄生虫活性的细胞因子网络的一部分。
