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聚合酶链反应:当前及未来的临床应用

The polymerase chain reaction: current and future clinical applications.

作者信息

Lynch J R, Brown J M

机构信息

Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford.

出版信息

J Med Genet. 1990 Jan;27(1):2-7. doi: 10.1136/jmg.27.1.2.

DOI:10.1136/jmg.27.1.2
PMID:2407845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1016870/
Abstract

The polymerase chain reaction has undergone rapid improvement since its initial development, such that the technique currently permits rapid, accurate, predictive tests to be made in the field of prenatal diagnosis and has greatly aided forensic medicine. It is anticipated that the polymerase chain reaction will also facilitate advances in other fields, in particular preimplantation diagnosis, virology, bacteriology, and cancer therapy.

摘要

聚合酶链反应自最初开发以来已取得迅速进展,以至于该技术目前能够在产前诊断领域进行快速、准确的预测性检测,并且极大地辅助了法医学。预计聚合酶链反应还将推动其他领域的进展,特别是植入前诊断、病毒学、细菌学和癌症治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/1016870/0d7beee81d5d/jmedgene00039-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/1016870/0d7beee81d5d/jmedgene00039-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df67/1016870/0d7beee81d5d/jmedgene00039-0013-a.jpg

相似文献

1
The polymerase chain reaction: current and future clinical applications.聚合酶链反应:当前及未来的临床应用
J Med Genet. 1990 Jan;27(1):2-7. doi: 10.1136/jmg.27.1.2.
2
Preimplantation diagnosis with the polymerase chain reaction.聚合酶链反应植入前诊断
BMJ. 1989 Jul 1;299(6690):3. doi: 10.1136/bmj.299.6690.3.
3
Detection of both the normal and mutant alleles in single cells of individuals heterozygous for the sickle cell mutation--prelude to preimplantation diagnosis.镰状细胞突变杂合个体单细胞中正常和突变等位基因的检测——植入前诊断的前奏。
Prenat Diagn. 1993 Jan;13(1):45-53. doi: 10.1002/pd.1970130107.
4
[Prenatal diagnosis of sickle cell anemia].[镰状细胞贫血的产前诊断]
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Preimplantation genetic diagnosis--counting chickens before they hatch?植入前基因诊断——是不是操之过急了?
Hum Reprod. 1992 May;7(5):583-4.
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Optimal polymerase chain reaction amplification for preimplantation diagnosis in cystic fibrosis (delta F508).用于囊性纤维化(ΔF508)植入前诊断的最佳聚合酶链反应扩增
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Detection of sickle cell anaemia and thalassaemias.镰状细胞贫血和地中海贫血的检测。
Nature. 1987;329(6137):293-4. doi: 10.1038/329293b0.
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Preimplantation genetics--two first steps.植入前遗传学——两个第一步。
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Rapid prenatal diagnosis of sickle cell diseases using oligonucleotide ligation assay coupled with laser-induced capillary fluorescence detection.使用寡核苷酸连接分析结合激光诱导毛细管荧光检测对镰状细胞疾病进行快速产前诊断。
Prenat Diagn. 2002 Aug;22(8):686-91. doi: 10.1002/pd.380.
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Strategies for preimplantation genetic diagnosis of single gene disorders by DNA amplification.通过DNA扩增进行单基因疾病植入前基因诊断的策略。
Prenat Diagn. 1998 Dec;18(13):1389-401.

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Mutation analysis in Turkish phenylketonuria patients.土耳其苯丙酮尿症患者的突变分析
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本文引用的文献

1
The molecular genetics of human hemoglobin.人类血红蛋白的分子遗传学
Prog Nucleic Acid Res Mol Biol. 1984;31:315-462. doi: 10.1016/s0079-6603(08)60382-7.
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Hypervariable 'minisatellite' regions in human DNA.人类DNA中的高变“微卫星”区域。
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Prevalence of ras gene mutations in human colorectal cancers.人类结直肠癌中ras基因突变的发生率。
Nature. 1987;327(6120):293-7. doi: 10.1038/327293a0.
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Direct cloning and sequence analysis of enzymatically amplified genomic sequences.酶促扩增基因组序列的直接克隆与序列分析
Science. 1986 Sep 5;233(4768):1076-8. doi: 10.1126/science.3461561.
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Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.通过聚合酶催化的链式反应在体外特异性合成DNA。
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Amplification and analysis of DNA sequences in single human sperm and diploid cells.单个人类精子和二倍体细胞中DNA序列的扩增与分析。
Nature. 1988 Sep 29;335(6189):414-7. doi: 10.1038/335414a0.
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Multiplying genes by leaps and bounds.
Science. 1988 Jun 10;240(4858):1408-10. doi: 10.1126/science.3375831.
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Detection of HIV-1 RNA sequences by in vitro DNA amplification.通过体外DNA扩增检测HIV-1 RNA序列。
Nucleic Acids Res. 1988 May 11;16(9):4165. doi: 10.1093/nar/16.9.4165.