Gaivelyte Kristina, Jakstas Valdas, Razukas Almantas, Janulis Valdimaras
Department of Pharmacognosy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Nat Prod Commun. 2013 Aug;8(8):1105-10.
Our research aim was to investigate the quantitative composition of neochlorogenic acid, chlorogenic acid, rutin, hyperoside and isoquercitrin in leaves and fruit of introduced rowan plants to Lithuania using a HPLC method. In leaf and fruit samples, quantitative estimation of neochlorogenic acid (in leaf samples 1.11-11.49 mg/g, in fruit samples 0.93-5.43 mg/g), chlorogenic acid (in leaf samples 2.74-21.91 mg/g, in fruit samples 0.55-7.50 mg/g), rutin (in leaf samples 0.03-4.88 mg/g, in fruit samples 0.02-0.39 mg/g), hyperoside (in leaf samples 0.15-7.44 mg/g, in fruit samples 0.02-1.19 mg/g) and isoquercitrin (in leaf samples 0.75-5.24 mg/g, in fruit samples 0.02-0.65 mg/g) was performed. In leaf samples, the total content of identified phenolic compounds ranged from 7.18 to 35.74 mg/g. In fruit, raw material total content of neochlorogenic acid, chlorogenic acid and identified flavonoids varied from 2.24 to 11.19 mg/g. Comparison of raw material samples of Sorbus species according to their quantitative composition of identified phenolic compounds using cluster analysis singularized S. commixta leaf and fruit samples. These contained the highest contents of chlorogenic acid, hyperoside and isoquercitrin, but also the lowest contents of neochlorogenic acid and rutin. The highest contents of neochlorogenic acid were recorded for S. lancifolia leaves, and of rutin for S. anglica leaves.
我们的研究目的是采用高效液相色谱法研究引入立陶宛的花楸属植物叶片和果实中新绿原酸、绿原酸、芦丁、金丝桃苷和异槲皮苷的定量组成。在叶片和果实样品中,对新绿原酸(叶片样品中为1.11 - 11.49毫克/克,果实样品中为0.93 - 5.43毫克/克)、绿原酸(叶片样品中为2.74 - 21.91毫克/克,果实样品中为0.55 - 7.50毫克/克)、芦丁(叶片样品中为0.03 - 4.88毫克/克,果实样品中为0.02 - 0.39毫克/克)、金丝桃苷(叶片样品中为0.15 - 7.44毫克/克,果实样品中为0.02 - 1.19毫克/克)和异槲皮苷(叶片样品中为0.75 - 5.24毫克/克,果实样品中为0.02 - 0.65毫克/克)进行了定量测定。在叶片样品中,已鉴定出的酚类化合物总含量在7.18至35.74毫克/克之间。在果实中,新绿原酸、绿原酸和已鉴定出的黄酮类化合物的原料总含量在2.24至11.19毫克/克之间。使用聚类分析根据已鉴定出的酚类化合物的定量组成对花楸属物种的原料样品进行比较,将混生花楸的叶片和果实样品区分开来。这些样品中绿原酸、金丝桃苷和异槲皮苷的含量最高,但新绿原酸和芦丁的含量最低。柳叶花楸叶片中记录到新绿原酸含量最高,而英国花楸叶片中芦丁含量最高。