Raudonis Raimondas, Raudonė Lina, Gaivelytė Kristina, Viškelis Pranas, Janulis Valdimaras
a Department of Pharmacognosy , Lithuanian University of Health Sciences , Mickeviciaus Str. 9, LT-44307 Kaunas , Lithuania.
Nat Prod Res. 2014;28(16):1231-40. doi: 10.1080/14786419.2014.895727. Epub 2014 Mar 17.
Fruits of different Sorbus L. species have been traditionally used in food production and in the treatment of various ailments. Besides common antioxidant active phytochemicals such as ascorbic acid, tocopherols and carotenoids, fruits are rich in phenolic compounds that are of particular interest. In this study, profiles of separate antioxidant active compounds (reducing and radical-scavenging activities) were investigated using online assays with post-column reactions. Significant variations in phenolic acids and flavonoids contents and antioxidant activity values were observed in the fruits of Sorbus L. species studied. Neochlorogenic and chlorogenic acids were determined as markers of antioxidant activity. Characteristic patterns of antioxidant profiles obtained using HPLC post-column FRAP and ABTS assays significantly depend on specific Sorbus L. species and are suitable for equivalency research of Sorbus L. fruits.
不同花楸属植物的果实传统上用于食品生产和治疗各种疾病。除了常见的具有抗氧化活性的植物化学物质,如抗坏血酸、生育酚和类胡萝卜素外,果实还富含特别引人关注的酚类化合物。在本研究中,使用柱后反应在线分析方法研究了单独的抗氧化活性化合物(还原和自由基清除活性)的概况。在所研究的花楸属植物果实中,观察到酚酸和黄酮类化合物含量以及抗氧化活性值存在显著差异。新绿原酸和绿原酸被确定为抗氧化活性的标志物。使用高效液相色谱柱后铁还原抗氧化能力(FRAP)和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)分析获得的抗氧化概况特征模式显著取决于特定的花楸属植物种类,适用于花楸属果实的等效性研究。