Wallet M A, Calderon N l, Alonso T R, Choe C S, Catalfamo D l, Lalane C J, Neiva K G, Panagakos F, Wallet S M
Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA.
Oral Dis. 2013 Apr;19(3):296-302. doi: 10.1111/odi.12001.
Periodontal diseases are a class of pathologies wherein oral microbes induce harmful immune responses in a susceptible host. Therefore, an agent that can both reduce microbial burden and lessen pathogenesis of localized inflammation would have beneficial effects in periodontal disease; 2,4,4-trichloro-2-hydroxydiphenyl-ether [triclosan] is currently used in oral care products owing to broad spectrum antimicrobial and anti-inflammatory properties.
To determine effects of triclosan on the response of oral epithelial cells to stimulation with the inflammatory microbial product lipopolysaccharide (LPS), a ligand for toll-like receptor 4 [TLR4].
MATERIALS/METHODS: Primary human oral epithelial cells were stimulated with LPS in the presence and/or absence of triclosan after which expression of pro-inflammatory cytokines, β-defensins, micro-RNAs [miRNAs], or TLR-signaling pathway proteins were evaluated.
Here, we demonstrate that triclosan is a potent inhibitor of oral epithelial cell LPS-induced pro-inflammatory responses by inducing miRNA regulation of the TLR-signaling pathway. Triclosan was not a pan-suppresser of oral epithelial cell responses as β-defensin 2 [βD2] and βD3 were upregulated by triclosan following LPS-stimulation.
These data demonstrate both a novel antimicrobial mechanism by which triclosan improves plaque control and an additional anti-inflammatory property, which could have beneficial effects in periodontal disease resolution.
牙周疾病是一类病理学病症，其中口腔微生物在易感宿主中引发有害的免疫反应。因此，一种既能减轻微生物负荷又能减轻局部炎症发病机制的药物对牙周疾病会有有益效果；2,4,4-三氯-2-羟基二苯醚[三氯生]由于具有广谱抗菌和抗炎特性，目前被用于口腔护理产品中。
确定三氯生对口腔上皮细胞对炎症性微生物产物脂多糖（LPS，一种Toll样受体4[TLR4]的配体）刺激的反应的影响。
材料/方法：在有和/或没有三氯生的情况下，用LPS刺激原代人口腔上皮细胞，然后评估促炎细胞因子、β-防御素、微小RNA[miRNA]或TLR信号通路蛋白的表达。
在此，我们证明三氯生通过诱导miRNA对TLR信号通路的调节，是口腔上皮细胞LPS诱导的促炎反应的有效抑制剂。三氯生不是口腔上皮细胞反应的泛抑制剂，因为在LPS刺激后，三氯生上调了β-防御素2[βD2]和βD3。
这些数据证明了三氯生改善牙菌斑控制的一种新的抗菌机制以及另一种抗炎特性，这可能对牙周疾病的解决有有益作用。
