Department of Physics, Korea Advanced Institute of Science and Technology , Daejeon 305-701, South Korea.
Anal Chem. 2013 Nov 5;85(21):10519-25. doi: 10.1021/ac402521u. Epub 2013 Oct 17.
We present a novel spectroscopic quantitative phase imaging technique with a wavelength swept-source, referred to as swept-source diffraction phase microscopy (ssDPM), for quantifying the optical dispersion of microscopic individual samples. Employing the swept-source and the principle of common-path interferometry, ssDPM measures the multispectral full-field quantitative phase imaging and spectroscopic microrefractometry of transparent microscopic samples in the visible spectrum with a wavelength range of 450-750 nm and a spectral resolution of less than 8 nm. With unprecedented precision and sensitivity, we demonstrate the quantitative spectroscopic microrefractometry of individual polystyrene beads, 30% bovine serum albumin solution, and healthy human red blood cells.
我们提出了一种新颖的光谱定量相衬成像技术，采用波长扫描光源，称为扫频源衍射相显微镜（ssDPM），用于量化微观单个样品的光色散。ssDPM 利用扫频源和共光路干涉原理，测量透明微观样品在可见光谱范围内（450-750nm）的多光谱全场定量相衬成像和光谱微折射，光谱分辨率小于 8nm。我们展示了单个聚苯乙烯珠、30%牛血清白蛋白溶液和健康人红细胞的定量光谱微折射，其具有前所未有的精度和灵敏度。
