Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Department of Pediatrics, Division of Pediatric Hematology/Oncology, Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta, Emory University School of Medicine, Atlanta, Georgia, USA.
Sci Rep. 2018 Jul 2;8(1):9913. doi: 10.1038/s41598-018-28208-0.
Ultraviolet (UV) spectroscopy is a powerful tool for quantitative (bio)chemical analysis, but its application to molecular imaging and microscopy has been limited. Here we introduce ultraviolet hyperspectral interferometric (UHI) microscopy, which leverages coherent detection of optical fields to overcome significant challenges associated with UV spectroscopy when applied to molecular imaging. We demonstrate that this method enables quantitative spectral analysis of important endogenous biomolecules with subcellular spatial resolution and sensitivity to nanometer-scaled structures for label-free molecular imaging of live cells.
紫外（UV）光谱学是定量（生物）化学分析的有力工具，但将其应用于分子成像和显微镜技术一直受到限制。在这里，我们引入了紫外高光谱干涉（UHI）显微镜技术，该技术利用光学场的相干检测来克服将 UV 光谱学应用于分子成像时所面临的重大挑战。我们证明，该方法能够以亚细胞空间分辨率对重要的内源性生物分子进行定量光谱分析，并对纳米级结构具有敏感性，从而实现活细胞的无标记分子成像。
