Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, PR China; Wenzhou Medical University, Wenzhou, Zhejiang 325035, PR China.
J Ethnopharmacol. 2013 Nov 25;150(2):507-13. doi: 10.1016/j.jep.2013.08.058. Epub 2013 Sep 28.
Flos Chrysanthemi is used in a variety of diseases in traditional Chinese medicine including hypertension, and the total flavonoids (rich in luteolin (LUT) and buddleoside (BUD)) of Flos Chrysanthemi is known to modulate vascular functions and reduce the blood pressure. However, the active flavonoids and their synergistic effects on anti-hypertension are still unclear. To investigate the combined anti-hypertension effects of LUT and BUD enriched extracts on spontaneously hypertensive rats (SHR), as well as the anti-hypertensive mechanism of LUT&BUD mixture.
CODA Mouse & Rat Tail-Cuff Blood Pressure System was used to measure the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of SHR after treated with extracts contains with LUT and/or BUD. The expressions of Ang II, PRA, ALD, ET, PGI2 and TXB2 were investigated by ELASA. Serum NO concentration was measured by the method of Nitric acid reductase.
A single administration of LUT, BUD, or LUT:BUD=1:1 significantly reduced SBP by about 3.35 mmHg, 4.39 mmHg and 15.42 mmHg, respectively. Chronic administration of LBM (at 60 mg/kg; p.o. for 30 days) reduced both SBP and DBP by 4.04% and 5.24% of the vehicle group, respectively. Oral administration of LBM at 60 mg/kg inhibited the serum levels of ANG, ALD and ET, but increased serum NO concentration.
This study shows the synergistic anti-hypertension effects of LUT and BUD in SHR. The effects of LBM on blood pressure are associated with RAAS and endothelial system. Thus, our experiments suggest that the combination of luteolin and buddleoside from Flos Chrysanthemi are potentially useful for the therapeutic treatments for hypertension.
菊花在中药中用于治疗多种疾病，包括高血压，菊花中的总黄酮（富含木樨草素（LUT）和川续断苷（BUD））被认为可以调节血管功能并降低血压。然而，活性黄酮及其对降压的协同作用仍不清楚。为了研究富含 LUT 和 BUD 的提取物对自发性高血压大鼠（SHR）的联合降压作用，以及 LUT&BUD 混合物的降压机制。
使用 CODA 小鼠和大鼠尾套血压系统测量经提取物处理后 SHR 的收缩压（SBP）和舒张压（DBP），提取物中含有 LUT 和/或 BUD。通过 ELISA 法测定 Ang II、PRA、ALD、ET、PGI2 和 TXB2 的表达。用硝酸还原酶法测定血清 NO 浓度。
单次给予 LUT、BUD 或 LUT:BUD=1:1 可分别使 SBP 降低约 3.35mmHg、4.39mmHg 和 15.42mmHg。慢性给予 LBM（60mg/kg；口服 30 天）使 SBP 和 DBP 分别降低 4.04%和 5.24%，与载体组相比。LBM 口服 60mg/kg 可抑制血清中 ANG、ALD 和 ET 的水平，但可增加血清中 NO 的浓度。
本研究表明 LUT 和 BUD 在 SHR 中具有协同降压作用。LBM 对血压的作用与 RAAS 和内皮系统有关。因此，我们的实验表明，菊花中的木樨草素和川续断苷的组合可能对高血压的治疗有一定的作用。
