Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 1524, CEP 05508-000, Sao Paulo, SP, Brazil.
Mar Environ Res. 2013 Dec;92:95-101. doi: 10.1016/j.marenvres.2013.09.005. Epub 2013 Sep 18.
Ocean temperatures are rising throughout the world, making it necessary to evaluate the impact of these temperature changes on sea urchins, which are well-known bioindicators. This study evaluated the effect of an increase in temperature on the immune response of the subtidal Lytechinus variegatus and the intertidal Echinometra lucunter sea urchins. Both species were exposed to 20 (control), 25 and 30 °C temperatures for 24 h, 2, 7 and 14 days. Counting of coelomocytes and assays on the phagocytic response, adhesion and spreading of coelomocytes were performed. Red and colorless sphere cells were considered biomarkers for heat stress. Moreover, a significant decrease in the phagocytic indices and a decrease in both cell adhesion and cell spreading were observed at 25 and 30 °C for L. variegatus. For E. lucunter, the only alteration observed was for the cell proportions. This report shows how different species of sea urchins respond immunologically to rising temperatures.
全球海洋温度不断上升，因此有必要评估这些温度变化对海胆（众所周知的生物指标）的影响。本研究评估了温度升高对潮间带 Lytechinus variegatus 和亚潮带 Echinometra lucunter 海胆免疫反应的影响。将这两个物种分别暴露于 20（对照）、25 和 30°C 的温度下 24 小时、2、7 和 14 天。对腔细胞计数和吞噬反应、腔细胞的黏附和铺展进行检测。红色和无色球体细胞被认为是热应激的生物标志物。此外，在 25 和 30°C 时，L. variegatus 的吞噬指数显著降低，并且细胞黏附和细胞铺展均降低。对于 E. lucunter，仅观察到细胞比例的变化。本报告展示了不同种类的海胆如何对升高的温度产生免疫反应。
