Pang Li, Wu Yang, Dong Ning, Xu Da-hai, Wang Da-wei, Wang Zhi-hao, Li Xing-liang, Bian Miao, Zhao Hui-jie, Liu Xiao-liang, Zhang Nan
Department of Emergency, The First Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Department of Respiratory, Qianwei Hospital of Jilin Province, Changchun, Jilin, PR China.
Clin Biochem. 2014 Jan;47(1-2):72-6. doi: 10.1016/j.clinbiochem.2013.09.015. Epub 2013 Sep 28.
Ubiquitin C-terminal hydrolase-L1 (UCH-L1) has been established as a reliable and potential biomarker of neuronal damage after acute neurologic insults, such as ischemic stroke, subarachnoid hemorrhage, and traumatic brain injury. However, the effect of serum UCH-L1 levels has not been investigated in carbon monoxide (CO)-poisoned patients. The aim of the present study was to evaluate whether serum UCH-L1 levels are a reliable marker of brain damage and the association of UCH-L1 with outcome.
This case-control study enrolled 46 CO-poisoned subjects and 30 controls. Using an enzyme-linked immunosorbent assay (ELISA) kit, we studied the temporal profile of serum UCH-L1 levels at 6, 12, 24 and 48 h after acute CO poisoning. Poisoning severity was assessed using the Glasgow Coma Scale (GCS) score. Long-term outcome was assessed using the Glasgow Outcome Scale (GOS) at 6 months after poisoning.
Compared with controls, CO-poisoned patients had significantly elevated serum levels of UCH-L1 at each time point after poisoning. There were significantly higher levels of UCH-L1 in CO-poisoned patients with a lower GCS score as well as in those with a poor 6-month outcome dichotomized GOS.
Serum levels of UCH-L1 appear to have potential clinical utility in providing valuable information about poisoning severity and outcome after CO poisoning.
泛素羧基末端水解酶-L1（UCH-L1）已被确立为急性神经损伤（如缺血性中风、蛛网膜下腔出血和创伤性脑损伤）后神经元损伤的可靠且有潜力的生物标志物。然而，尚未对一氧化碳（CO）中毒患者血清UCH-L1水平的影响进行研究。本研究的目的是评估血清UCH-L1水平是否为脑损伤的可靠标志物以及UCH-L1与预后的关联。
本病例对照研究纳入了46名CO中毒受试者和30名对照。使用酶联免疫吸附测定（ELISA）试剂盒，我们研究了急性CO中毒后6、12、24和48小时血清UCH-L1水平的时间变化情况。使用格拉斯哥昏迷量表（GCS）评分评估中毒严重程度。在中毒后6个月使用格拉斯哥预后量表（GOS）评估长期预后。
与对照组相比，CO中毒患者在中毒后各时间点的血清UCH-L1水平均显著升高。GCS评分较低的CO中毒患者以及6个月预后不良（根据GOS二分法）的患者中UCH-L1水平显著更高。
血清UCH-L1水平似乎在提供有关CO中毒严重程度和预后的有价值信息方面具有潜在的临床应用价值。
