Guffanti A A, Corpe W A
J Bacteriol. 1975 Oct;124(1):262-8. doi: 10.1128/jb.124.1.262-268.1975.
Pseudomonas fluorescens W uses maltose exclusively by hydrolyzing it to glucose via an inducible alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20). No evidence for phosphorolytic cleavage or oxidation to maltobionic acid was found in this organism. The alpha-glucosidase was totally intracellular and was most active at pH of 7.0. Induction occurred when cells were incubated with maltotriose or maltose. Induction was rapid and easily detectable within the first 5 min after the addition of the inducer. Glucose and its derivatives did not repress induction. Cells growing on DL-alanine or succinate plus maltose exhibited lower levels of alpha-glucosidase than those grown on maltose alone or maltose plus glucose. Induction required both messenger ribonucleic acid and protein synthesis.
荧光假单胞菌W仅通过一种可诱导的α-葡萄糖苷酶（α-D-葡萄糖苷葡糖水解酶，EC 3.2.1.20）将麦芽糖水解为葡萄糖来利用麦芽糖。在该生物体中未发现磷酸解裂解或氧化为麦芽酮糖酸的证据。α-葡萄糖苷酶完全位于细胞内，在pH 7.0时活性最高。当细胞与麦芽三糖或麦芽糖一起孵育时会发生诱导。诱导迅速，在添加诱导剂后的最初5分钟内即可轻松检测到。葡萄糖及其衍生物不会抑制诱导。在DL-丙氨酸或琥珀酸盐加麦芽糖上生长的细胞，其α-葡萄糖苷酶水平低于仅在麦芽糖或麦芽糖加葡萄糖上生长的细胞。诱导需要信使核糖核酸和蛋白质合成。
