Sugimoto T, Amemura A, Harada T
Appl Microbiol. 1974 Sep;28(3):336-9. doi: 10.1128/am.28.3.336-339.1974.
Pseudomonas SB15, which produces extracellular isoamylase, was found to produce intracellular alpha-glucosidase and amylase(s) when grown on maltose. A mutant strain (MS1) derived from it, which formed isoamylase constitutively, also produced these intracellular enzymes constitutively. The activities of the enzymes produced in the mutant strain were much greater than those induced in the parent strain.
产细胞外异淀粉酶的假单胞菌SB15在以麦芽糖为碳源生长时,被发现可产生细胞内α-葡萄糖苷酶和淀粉酶。从该菌株衍生出的一个突变株(MS1),其组成型表达异淀粉酶,同时也组成型表达这些细胞内酶。突变株中产生的这些酶的活性比亲本菌株中诱导产生的酶的活性高得多。