Rice G E, Smirnis M
Department of Physiology, Monash University, Clayton, Victoria, Australia.
Regul Pept. 1990 Jan;27(1):51-9. doi: 10.1016/0167-0115(90)90204-a.
Luteinizing hormone releasing hormone (LHRH)-like immunoactivity has been identified in cotyledonary extracts prepared from pregnant ewes. This activity displayed similar physico-chemical properties as synthetic LHRH, as determined by reverse-phase HPLC and size-exclusion HPLC. Under reverse-phase conditions, cotyledonary LHRH-like immunoactivity displayed a retention time (10.5 +/- 0.1 min) which was not significantly different from that of synthetic LHRH. When subjected to size-exclusion HPLC, cotyledonary LHRH-like immunoactivity eluted in fractions which corresponded to a molecular weight range of 1100-1200 Da, which was not significantly different from the elution profile observed for synthetic LHRH. The cotyledonary tissue content of LHRH-like immunoactivity averaged 94 +/- 24 pg/mg (n = 6). The results of this study demonstrate the presence of LHRH-like immunoactivity in ovine cotyledons. Although placental synthesis of LHRH-like immunoactive material has been demonstrated in other species, it remains to be established whether this activity, demonstrated in ovine placenta, is the consequence of de novo placental synthesis or represents uptake from the maternal (and/or fetal) circulation.
在从怀孕母羊制备的子叶提取物中已鉴定出促黄体生成激素释放激素（LHRH）样免疫活性。通过反相高效液相色谱法（HPLC）和尺寸排阻HPLC测定，这种活性表现出与合成LHRH相似的物理化学性质。在反相条件下，子叶LHRH样免疫活性的保留时间为（10.5±0.1分钟），与合成LHRH的保留时间无显著差异。当进行尺寸排阻HPLC时，子叶LHRH样免疫活性在对应于分子量范围为1100 - 1200 Da的级分中洗脱，这与合成LHRH观察到的洗脱谱无显著差异。子叶组织中LHRH样免疫活性的含量平均为94±24 pg/mg（n = 6）。本研究结果证明了绵羊子叶中存在LHRH样免疫活性。尽管在其他物种中已证明胎盘能合成LHRH样免疫活性物质，但在绵羊胎盘中证明的这种活性是胎盘从头合成的结果还是代表从母体（和/或胎儿）循环中摄取，仍有待确定。
