Wheaton J E
Horm Metab Res. 1980 Jul;12(7):314-7. doi: 10.1055/s-2007-996278.
Ovine pineal glands (PGs, n = 168), cerebral cortex(C) and stalk-median eminences (SMEs) were obtained fresh from slaughterhouse sheep and mature ewes during the estrous cycle. Tissues were extracted in 2N acetic acid (aa) followed by either glacial aa or ethanol. Portions of the preparations were further subjected to gel filtration using Sephadex G-25. Neutralized extracts were assayed for LHRH using radioimmunoassay. LHRH immunoreactivity was consistantly detected in the various extracts, ranging from 7-150 pg/PG (0.1-1.7 pg/mg), 0.1-0.3 pg/mg C and 35-72 ng/SME (0.7-1.4 ng/mg). Slopes of binding displacement regression lines for the preparations were not different from that of synthetic LHRH. Chromatographic elution profiles for preparations of SMEs and PGs were similar and the bulk of immunoreactivity eluted in a position coincident with that of synthetic LHRH. Immunoreactivity in cortical extracts ran in advance of the others. PG contents of LHRH immunoreactivity were similar during the estrous cycle.
在发情周期期间，从屠宰场的绵羊和成年母羊新鲜获取168个绵羊松果体（PGs）、大脑皮层（C）和柄 - 正中隆起（SMEs）。将组织在2N乙酸（aa）中提取，随后用冰乙酸或乙醇处理。部分制剂进一步使用葡聚糖凝胶G - 25进行凝胶过滤。使用放射免疫分析法测定中和提取物中的促性腺激素释放激素（LHRH）。在各种提取物中均持续检测到LHRH免疫反应性，范围为7 - 150 pg/PG（0.1 - 1.7 pg/mg）、0.1 - 0.3 pg/mg C和35 - 72 ng/SME（0.7 - 1.4 ng/mg）。制剂的结合置换回归线斜率与合成LHRH的斜率无差异。SMEs和PGs制剂的色谱洗脱图谱相似，大部分免疫反应性在与合成LHRH一致的位置洗脱。皮质提取物中的免疫反应性比其他提取物提前出现。发情周期期间，PGs中LHRH免疫反应性含量相似。
