Morsy Gamal M, Abou El-Ala Kawther S, Ali Atef A
Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt
Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt.
Toxicol Ind Health. 2016 Feb;32(2):200-14. doi: 10.1177/0748233713498462. Epub 2013 Sep 30.
The present work aimed to evaluate the oxidative stress of nanoalumina (aluminium oxide nanoparticles, Al2O3-NPs) with a diameter <13 nm (9.83 ± 1.61 nm) as assessed by the perturbations in the enzymatic and non-enzymatic antioxidants as well as lipid peroxidation (LPO) in the brain, liver and kidney of male albino rats, after 2 days of single acute dose (3.9 or 6.4 or 8.5 g/kg) injection and a sublethal dose of 1.3 g/kg once in 2 days for a period of 28 days. According to two-way analysis of variance, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities as well as the levels of glutathione (GSH) and LPO were significantly affected by the injected doses, organs and their interactions. On the other hand, in sublethal experiments, these parameters were affected by the experimental periods, organs and their interactions. Regression analysis confirmed that the activities of SOD, CAT, GPx and GSH levels in the brain, liver and kidney were inversely proportional with the acute doses, the experimental periods, and aluminium accumulated in these tissues, whereas the levels of LPO exhibited a positive relationship. Correlation coefficient indicated that oxidative stress mainly depends on aluminium accumulated in the studied organs, followed by injected doses and the experimental periods. In comparison with the corresponding controls, the acute and sublethal doses of Al2O3-NPs caused significant inhibition of the brain, hepatic and renal SOD, CAT, GPx activities and a severe marked reduction in the concentrations of GSH that were associated with a significant elevation in the levels of malondialdehyde (an indicator of LPO). In conclusion, our data indicated that rats injected with nanoalumina suffered from the oxidative stresses that were dose and time dependent. In addition, Al2O3-NPs released into the biospheres could be potentiating a risk to the environment and causing hazard effects on living organisms, including mammals.
本研究旨在评估直径<13 nm（9.83±1.61 nm）的纳米氧化铝（氧化铝纳米颗粒，Al2O3-NPs）的氧化应激，通过雄性白化大鼠脑、肝和肾中酶促和非酶促抗氧化剂的扰动以及脂质过氧化（LPO）来评估，在单次急性剂量（3.9或6.4或8.5 g/kg）注射2天后，以及在28天内每2天一次给予1.3 g/kg的亚致死剂量后进行评估。根据双向方差分析，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性以及谷胱甘肽（GSH）和LPO水平受到注射剂量、器官及其相互作用的显著影响。另一方面，在亚致死实验中，这些参数受到实验周期、器官及其相互作用的影响。回归分析证实，脑、肝和肾中SOD、CAT、GPx活性和GSH水平与急性剂量、实验周期以及这些组织中积累的铝呈反比，而LPO水平呈正相关。相关系数表明，氧化应激主要取决于所研究器官中积累的铝，其次是注射剂量和实验周期。与相应对照组相比，Al2O3-NPs的急性和亚致死剂量导致脑、肝和肾SOD、CAT、GPx活性显著抑制，GSH浓度严重显著降低，同时丙二醛（LPO的指标）水平显著升高。总之，我们的数据表明，注射纳米氧化铝的大鼠遭受了剂量和时间依赖性的氧化应激。此外，释放到生物圈中的Al2O3-NPs可能会增加对环境的风险，并对包括哺乳动物在内的生物造成危害。
