Higuchi Hidetoshi, Ito Erina, Iwano Hidetoma, Oikawa Shin, Nagahata Hajime
Animal Health Laboratory (Higuchi, Ito, Nagahata), Department of Veterinary Herd Health (Oikawa), Department of Health and Environmental Sciences and Veterinary Biochemistry, and Department of Bioscience (Iwano), School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Can J Vet Res. 2013 Apr;77(2):120-5.
The effects of vitamin E supplementation on cellular α-tocopherol concentrations of neutrophils from Holstein calves and the mechanism of scavenger receptor class B type I (SR-BI)-mediated uptake of α-tocopherol were examined. Cellular α-tocopherol concentrations in vitamin E-treated calves increased from 3.5 ± 0.38 to 7.2 ± 0.84 μg/10(7) cells, respectively, within 14 d after vitamin E supplementation; these concentrations were significantly higher than those of control calves (P < 0.01). The expression indices of SR-BI [a major receptor that recognizes high-density lipoprotein (HDL)] mRNA in neutrophils were two to five times higher (P < 0.01) in neutrophils obtained from vitamin E-supplemented calves compared with those from control calves, and anti-SR-B1 antibody, ranging from 0.1 to 1.0 μg/mL, significantly (P < 0.01) decreased cellular α-tocopherol concentrations of neutrophils. Cytochalasin D and latrunculin B, major inhibitors of actin polymerization of neutrophils, significantly decreased cellular α-tocopherol concentrations of neutrophils (P < 0.01). Our results demonstrated that in vitamin E-supplemented calves: 1) α-tocopherol is mainly distributed with HDL, 2) α-tocopherol within HDL is recognized by SR-BI on the surface of neutrophils, and 3) rearrangement of the actin cytoskeleton is a crucial step for the uptake of α-tocopherol by neutrophils.
研究了补充维生素E对荷斯坦犊牛中性粒细胞中细胞α-生育酚浓度的影响以及I型清道夫受体（SR-BI）介导的α-生育酚摄取机制。补充维生素E后14天内，维生素E处理的犊牛细胞α-生育酚浓度分别从3.5±0.38μg/10⁷细胞增加到7.2±0.84μg/10⁷细胞；这些浓度显著高于对照犊牛（P<0.01）。与对照犊牛相比，补充维生素E的犊牛中性粒细胞中SR-BI（一种识别高密度脂蛋白（HDL）的主要受体）mRNA的表达指数高2至5倍（P<0.01），浓度范围为0.1至1.0μg/mL的抗SR-B1抗体显著降低了中性粒细胞的细胞α-生育酚浓度（P<0.01）。细胞松弛素D和拉特罗毒素B是中性粒细胞肌动蛋白聚合的主要抑制剂，显著降低了中性粒细胞的细胞α-生育酚浓度（P<0.01）。我们的结果表明，在补充维生素E的犊牛中：1）α-生育酚主要与HDL一起分布，2）HDL中的α-生育酚被中性粒细胞表面的SR-BI识别，3）肌动蛋白细胞骨架的重排是中性粒细胞摄取α-生育酚的关键步骤。
