Thomsen H, Schulz A, Bhakdi S
Abteilung Rechtsmedizin, Christian-Albrechts-Universität zu Kiel.
Z Rechtsmed. 1990;103(3):199-206. doi: 10.1007/BF00207341.
With conventional staining techniques it was nearly impossible to differentiate with certainty between early ischemic myocardial lesions, autolytic changes and intact myocardium without a nuclear section. In 1986 it was introduced an immunohistochemical method, which presented C5b-9 Complement Complex as a necrosis marker in cryostat sections. Our further development of this process has resulted in a technique for application on routine paraffin sections: C5b-9 Complement Complex is particularly well-suited for the exact and autolytically insensitive presentation of disseminated necroses of fiber bundels and single fibers of myocardium. The application of the technique on paraffin sections will lead to the use of the same in forensic medicine routine diagnostics of early ischemic myocardial lesions, even in cases of "sudden" cardiac death.
使用传统染色技术时，若没有细胞核切片，几乎不可能准确区分早期缺血性心肌病变、自溶变化和完整的心肌。1986年引入了一种免疫组织化学方法，该方法在低温恒温器切片中呈现C5b - 9补体复合物作为坏死标志物。我们对这一方法的进一步改进产生了一种可应用于常规石蜡切片的技术：C5b - 9补体复合物特别适合于准确且对自溶不敏感地呈现心肌纤维束和单根纤维的弥漫性坏死。该技术在石蜡切片上的应用将使其可用于法医学中早期缺血性心肌病变的常规诊断，即使在“猝死”病例中也是如此。
