Datta M C
Prostaglandins. 1985 Apr;29(4):561-77. doi: 10.1016/0090-6980(85)90080-2.
The effects of prostaglandin E2 (PGE2) in association with erythropoietin on the synthesis of fetal and adult hemoglobin in peripheral blood-derived erythroid burst colonies from normal adults and from patients with sickle cell anemia were investigated. The synthesized hemoglobin at the end of 8, 14 or 18 days in culture was separated by DEAE-cellulose chromatography of 35S-methionine labelled hemoglobin. Quantitative estimation of the synthesized hemoglobin phenotypes, for the three indicated culture periods, showed preferential synthesis of Hb F in addition to an overall increase in hemoglobin synthesis in PGE2 treated colonies. Furthermore, the reactivation of fetal hemoglobin production by PGE2 was more pronounced when the adherent cells were included in the culture dishes. These results indicate that the addition of PGE2 to culture dishes presumably constitutes an environmental change to promote the functional changes seen in the blood erythroid bursts in terms of Hb synthesis and switching.
研究了前列腺素E2（PGE2）与促红细胞生成素联合使用对正常成年人及镰状细胞贫血患者外周血来源的红系爆式集落中胎儿血红蛋白和成人血红蛋白合成的影响。培养8天、14天或18天后合成的血红蛋白，通过对35S-甲硫氨酸标记的血红蛋白进行DEAE-纤维素色谱法分离。对三个指定培养期合成的血红蛋白表型进行定量估计，结果显示，除了PGE2处理的集落中血红蛋白合成总体增加外，还优先合成了Hb F。此外，当培养皿中包含贴壁细胞时，PGE2对胎儿血红蛋白产生的再激活作用更为明显。这些结果表明，向培养皿中添加PGE2可能构成一种环境变化，以促进在血液红系爆式集落中观察到的血红蛋白合成和转换方面的功能变化。
