Lavrijsen K L, Verwilghen R L
Biochim Biophys Acta. 1984 Apr 16;803(4):290-301. doi: 10.1016/0167-4889(84)90120-4.
When fetal calf liver erythroid cells were incubated in the presence of small amounts of progesterone (10(-7)-10(-8) M), the hemoglobin synthesis in these cells was significantly increased. The increase in the amount of radioactivity in de novo synthesized hemoglobins could be demonstrated when techniques such as isoelectric focusing, chromatography on DEAE-cellulose and gel chromatography on Sephadex G-100 were used to isolate the hemoglobin fraction. Using the latter technique, it was shown that the synthesis of cytoplasmic non-hemoglobin proteins in erythroid-cell lysates was also stimulated by progesterone. The presence of hepatocytes in culture nullified the hormone action. It was necessary that progesterone was present during the first hours of culture. Delayed addition of the steroid to the cells had no effect on hemoglobin synthesis. Erythropoietin was necessary to obtain stimulation by progesterone. These results suggest that the target cell of the hormone is an erythropoietin-sensitive cell. High concentrations of progesterone (10(-4) M) strongly inhibited hemoglobin synthesis in fetal calf erythroid cells. Culture of cells under this condition, however, gives rise to a cell population that preferentially synthesizes adult hemoglobin. Our results suggest that in the erythropoietic calf liver, high concentrations of progesterone may preferentially stimulate adult hemoglobin synthesis, or that those cells which have a high capacity to synthesize adult hemoglobins are less sensitive to toxic concentrations of the hormone. The effects of stimulation of hemoglobin synthesis in fetal calf erythroid cells occur at hormone concentrations that suggest a possible physiological role of progesterone in fetal, and eventually also in maternal, erythropoiesis.
当胎牛肝脏红系细胞在少量孕酮（10⁻⁷ - 10⁻⁸ M）存在的情况下进行孵育时，这些细胞中的血红蛋白合成显著增加。当使用诸如等电聚焦、DEAE - 纤维素柱色谱和Sephadex G - 100凝胶色谱等技术分离血红蛋白组分时，可以证明从头合成的血红蛋白中放射性量的增加。使用后一种技术表明，孕酮也能刺激红系细胞裂解物中细胞质非血红蛋白蛋白质的合成。培养中的肝细胞会抵消激素的作用。孕酮必须在培养的最初几个小时内存在。向细胞中延迟添加该类固醇对血红蛋白合成没有影响。促红细胞生成素是孕酮发挥刺激作用所必需的。这些结果表明，该激素的靶细胞是对促红细胞生成素敏感的细胞。高浓度的孕酮（10⁻⁴ M）强烈抑制胎牛红系细胞中的血红蛋白合成。然而，在此条件下培养细胞会产生优先合成成人血红蛋白的细胞群体。我们的结果表明，在造血的小牛肝脏中，高浓度的孕酮可能优先刺激成人血红蛋白的合成，或者那些具有高合成成人血红蛋白能力的细胞对该激素的毒性浓度不太敏感。在胎牛红系细胞中刺激血红蛋白合成的作用发生在激素浓度下，这表明孕酮在胎儿，最终也在母体的红细胞生成中可能具有生理作用。
