Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, via del Taglio 10, 43126 Parma, Italy.
Vet J. 2013 Nov;198(2):534-7. doi: 10.1016/j.tvjl.2013.08.020. Epub 2013 Aug 27.
The emergence of multidrug resistant (MDR) and extensively drug resistant (XDR) bacteria has become a medical and veterinary problem. Antimicrobial peptides (AMPs) show potential to overcome antibiotic resistance and could be used therapeutically. A novel AMP (AMP2041) was developed in silico and its microbiocidal activity against MDR clinical strains isolated from cattle (n=6), dogs (n=8), and pigs (n=20) was evaluated. AMP2041 showed strong antimicrobial activity against all Gram-positive and Gram-negative MDR clinical strains tested. Within 20 min of incubation, there was complete killing of Pseudomonas aeruginosa ATCC 27953 and a 90% reduction of colony count for Escherichia coli ATCC 25922. For Staphylococcus aureus ATCC 25923, a 90% reduction of colony count was observed within 120 min of incubation.
多药耐药（MDR）和广泛耐药（XDR）细菌的出现已经成为一个医学和兽医问题。抗菌肽（AMPs）显示出克服抗生素耐药性的潜力，并可用于治疗。本文在计算机上设计了一种新型抗菌肽（AMP2041），并评估了其对从牛（n=6）、狗（n=8）和猪（n=20）分离的 MDR 临床株的杀菌活性。AMP2041 对所有革兰氏阳性和革兰氏阴性 MDR 临床株均表现出较强的抗菌活性。孵育 20 分钟内，完全杀死铜绿假单胞菌 ATCC 27953，大肠杆菌 ATCC 25922 的菌落计数减少 90%。孵育 120 分钟内，金黄色葡萄球菌 ATCC 25923 的菌落计数减少 90%。
