Jarosiewicz Małgorzata, Garbacz Katarzyna, Neubauer Damian, Kamysz Wojciech
Department of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, 25 Dębowa Str., 80-204 Gdansk, Poland.
Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdansk, 107 Gen. J. Hallera Str., 80-416 Gdansk, Poland.
Animals (Basel). 2020 Mar 11;10(3):470. doi: 10.3390/ani10030470.
The emergence of staphylococcal canine pathogens resistant to multiple antimicrobial agents is a growing and urgent problem in veterinary practice. Antimicrobial peptides (AMPs) seem to be a promising alternative to conventional antibiotics. The aim of this in vitro study was to evaluate the antimicrobial activity of selected AMPs against pathogenic staphylococcal strains, including multidrug- and methicillin-resistant strains isolated from canine pyoderma cases. Seven antimicrobial peptides (aurein 1.2, CAMEL, citropin 1.1, protegrin-1, pexiganan, temporin A and uperin 3.6) synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase method were tested. The minimal inhibitory and minimal bactericidal concentrations (MIC and MBC) were determined by the broth microdilution method. The study showed that analyzed AMPs exerted an extensive effect against canine pathogens, with the most active peptide being uperin 3.6. The tested AMPs were equally efficient against both resistant- and susceptible staphylococcal strains and were more efficient against S than against strains. Our findings are particularly interesting from a clinical perspective, as they point to AMPs as potential therapeutic topical agents in canine pyoderma cases associated with antimicrobial resistance of staphylococci.
对多种抗菌药物耐药的葡萄球菌犬类病原体的出现，在兽医实践中是一个日益严重且紧迫的问题。抗菌肽（AMPs）似乎是传统抗生素的一种有前景的替代品。这项体外研究的目的是评估选定的抗菌肽对致病性葡萄球菌菌株的抗菌活性，包括从犬脓皮病病例中分离出的多重耐药和耐甲氧西林菌株。测试了通过9-芴甲氧羰基（Fmoc）固相法合成的七种抗菌肽（奥瑞因1.2、骆驼抗菌肽、柑橘肽1.1、防御素-1、派罗西汀、颞叶素A和uperin 3.6）。通过肉汤微量稀释法测定最小抑菌浓度和最小杀菌浓度（MIC和MBC）。研究表明，所分析的抗菌肽对犬类病原体具有广泛的作用，其中活性最强的肽是uperin 3.6。测试的抗菌肽对耐药和敏感葡萄球菌菌株的效果相同，且对S菌株的效果比对 菌株的效果更好。从临床角度来看，我们的发现特别有趣，因为它们指出抗菌肽作为与葡萄球菌抗菌耐药性相关的犬脓皮病病例中的潜在治疗外用剂。
