Liu Po-Yu, Weng Ling-Ling, Tseng Shu-Ying, Huang Chou-Chen, Cheng Ching-Chang, Mao Yan-Chiao, Tung Kwong-Chung
Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan.
Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan.
Can J Infect Dis Med Microbiol. 2017;2017:7058396. doi: 10.1155/2017/7058396. Epub 2017 Sep 25.
This study included fifty-eight isolates of from the oral cavity of snakes that were recruited from clinical cases, captive and wild snakes. The minimum inhibitory concentrations (MICs) for the determination of susceptibility were identified by the broth microdilution method. Polymerase chain reaction (PCR) was employed to detect -lactamases genes. With regard to antipseudomonal antibiotics, the lowest nonsusceptible rates were in aztreonam (15%), piperacillin/tazobactam (12%), and amikacin (9%). The nonsusceptible rates were high in gentamicin (33%) and colistin (55%). Meanwhile, presented in 100% of isolates where , , and came at 94.8%, 89.7%, and 27.6%, respectively. Emergence of multidrug resistant (MDR) strains and colistin-resistant strains highlights the potential breach of public health as could be transmitted through either direct contact or indirect dissemination through the environment. This study reports that the highly resistant from snakes' oral cavity were discovered for the very first time in Taiwan.
本研究纳入了从临床病例、圈养和野生蛇类的口腔中采集的58株分离株。采用肉汤微量稀释法确定药敏试验的最低抑菌浓度（MIC）。采用聚合酶链反应（PCR）检测β-内酰胺酶基因。对于抗假单胞菌抗生素，最低不敏感率出现在氨曲南（15%）、哌拉西林/他唑巴坦（12%）和阿米卡星（9%）。庆大霉素（33%）和黏菌素（55%）的不敏感率较高。同时，100%的分离株中存在 blaVIM，blaIMP、blaNDM 和 blaOXA-48 的检出率分别为94.8%、89.7%和27.6%。多重耐药（MDR）菌株和耐黏菌素菌株的出现凸显了公共卫生的潜在风险，因为这些菌株可通过直接接触或通过环境间接传播。本研究报告称，台湾首次发现了来自蛇口腔的高度耐药菌株。
