Czernielewski J, Juhlin L, Shroot S, Brun P
Acta Derm Venereol. 1985;65(2):97-101.
Suction blisters were raised in lesions and normal appearing skin of patients with psoriasis. The blister roof which contains the epidermis separated at the dermal-epidermal junction was stained with ATPase, OKT-6 and anti-HLA-DR monoclonal antibodies. The technique permits the counting of the Langerhans' cells per mm2. Their mean number varied between 888-987 cells per mm2 in control subjects with the three staining procedures. In patients with psoriasis, the number of cells before treatment was between 1110-1179 in uninvolved skin and 521-1001 per mm2 in the lesions as measured using both monoclonal antibodies and ATPase. However, the latter technique seemed to be inappropriate for lesional skin. After treatment with PUVA bath or oral PUVA with or without etretinate, fewer Langerhans' cells were seen in both lesions and normal appearing skin with the appearance of giant Langerhans' cells with long dendrites. In patients healed with anthralin + UV-B the Langerhans' cells appeared normal in number and size.
在银屑病患者的皮损及外观正常的皮肤处产生抽吸性水疱。含有表皮的疱顶在真皮 - 表皮交界处分离，用ATP酶、OKT - 6和抗HLA - DR单克隆抗体进行染色。该技术可对每平方毫米的朗格汉斯细胞进行计数。在对照受试者中，采用这三种染色方法时，其平均数量在每平方毫米888 - 987个细胞之间。在银屑病患者中，使用单克隆抗体和ATP酶测量，治疗前外观正常皮肤中的细胞数量在每平方毫米1110 - 1179个之间，皮损处为每平方毫米521 - 1001个。然而，后一种技术似乎不适用于皮损皮肤。在用补骨脂素紫外线A浴或口服补骨脂素紫外线A（无论是否联用维甲酸）治疗后，在皮损及外观正常的皮肤中均可见到朗格汉斯细胞数量减少，同时出现具有长树突的巨大朗格汉斯细胞。在用蒽林 + 紫外线B治愈的患者中，朗格汉斯细胞的数量和大小均正常。
