Oxholm A, Oxholm P, Staberg B
Acta Derm Venereol. 1987;67(1):8-11.
The Langerhans' cell (LC) density is known to be reduced in skin lesions as compared to uninvolved skin in patients with psoriasis. It is, however, still unsettled whether the LC density in uninvolved psoriatic skin differs from the density in normal skin. We have enumerated epidermal LC in uninvolved skin from 15 patients with stable psoriasis and in 15 healthy subjects. Punch biopsies from non-sunexposed skin from the buttock were taken. Epidermal sheets were separated by EDTA and LC then stained with an indirect immunoperoxidase technique using the mouse monoclonal antibody OKT6. The LC density was significantly reduced in uninvolved skin of patients with psoriasis (mean +/- SD: 375 +/- 37/mm2) as compared to healthy controls (544 +/- 168/mm2) (p less than 0.01). A reduced number of LC in uninvolved psoriatic skin is in accordance with previous reports demonstrating an impaired DNCB reactivity in patients with psoriasis. Whether the reduction in LC density is of pathogenic importance for psoriasis is unknown.
已知与银屑病患者未受累皮肤相比，其皮肤损害处的朗格汉斯细胞（LC）密度降低。然而，未受累银屑病皮肤中的LC密度是否与正常皮肤中的密度不同仍未明确。我们已对15例病情稳定的银屑病患者和15名健康受试者的未受累皮肤中的表皮LC进行了计数。从臀部未暴露于阳光的皮肤处取打孔活检标本。用乙二胺四乙酸分离表皮片，然后使用小鼠单克隆抗体OKT6通过间接免疫过氧化物酶技术对LC进行染色。与健康对照（544±168/mm²）相比，银屑病患者未受累皮肤中的LC密度显著降低（均值±标准差：375±37/mm²）（p＜0.01）。未受累银屑病皮肤中LC数量减少与先前报道一致，先前报道表明银屑病患者二硝基氯苯反应性受损。LC密度降低对银屑病是否具有致病重要性尚不清楚。
