Kishikawa Hiroaki, Ebberyd Anette, Römling Ute, Brauner Annelie, Lüthje Petra, Lundberg Jon O, Weitzberg Eddie
Department of Physiology and Pharmacology, Karolinska Institutet, S-171 77 Stockholm, Sweden.
Department of Microbiology, Tumor Biology, and Cell Biology, Karolinska Institutet, S-171 77 Stockholm, Sweden.
Free Radic Biol Med. 2013 Dec;65:1257-1264. doi: 10.1016/j.freeradbiomed.2013.09.012. Epub 2013 Sep 29.
Antibacterial nitrogen oxides including nitric oxide are formed from nitrite under acidic conditions. In a continuous-flow model of the urinary bladder we used the retention cuff of an all-silicone Foley catheter as a depot for export of nitrogen oxides. The cuff was filled with sodium nitrite (50mM) and an acidic buffer solution (pH 3.6) and the growth of nine common uropathogens in the surrounding artificial urine was measured along with biofilm formation on the catheter surface. In experiments with control catheters (NaCl) bacteria grew readily and biofilm developed within hours in five of nine strains. In contrast, with test catheters bacterial counts were markedly reduced and biofilm formation by Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterobacter cloace was prevented, whereas Escherichia coli and Staphylococcus aureus were unaffected. We conclude that antibacterial nitrogen oxides generated in the retention cuff of a urinary catheter diffuse into urine and prevent the growth of urinary pathogens and biofilm formation. Although promising, future studies will reveal if this novel approach can be clinically useful for the prevention of catheter-associated urinary tract infections.
包括一氧化氮在内的抗菌性氮氧化物在酸性条件下由亚硝酸盐形成。在膀胱的连续流模型中，我们使用全硅胶导尿管的潴留袖套作为氮氧化物输出的储存库。将袖套填充亚硝酸钠（50mM）和酸性缓冲溶液（pH 3.6），并测量周围人工尿液中九种常见尿路致病菌的生长情况以及导管表面生物膜的形成情况。在对照导管（NaCl）的实验中，细菌易于生长，9种菌株中有5种在数小时内形成生物膜。相比之下，使用测试导管时细菌计数显著降低，铜绿假单胞菌、肺炎克雷伯菌和阴沟肠杆菌的生物膜形成受到抑制，而大肠杆菌和金黄色葡萄球菌未受影响。我们得出结论，导尿管潴留袖套中产生的抗菌性氮氧化物扩散到尿液中，可防止尿路致病菌生长和生物膜形成。尽管前景广阔，但未来研究将揭示这种新方法在临床上是否可用于预防导管相关尿路感染。
