Zhang Yaqian, Zhao Yulin, Dong Dong, Li Xiaoping, Li Zhi, Li Siyu, Wang Juan
Department of Rhinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Exp Ther Med. 2020 Feb;19(2):1267-1274. doi: 10.3892/etm.2019.8344. Epub 2019 Dec 18.
() is associated with recalcitrant chronic infection, especially in chronic rhinosinusitis (CRS). infection and biofilms cause poorer postsurgical outcomes. We developed isosorbide mononitrate (ISMN) loaded nanoparticles conjugated with an anti- alpha-toxin (anti- α-toxin) antibody that could target biofilms and investigated their anti-biofilm effect. Anti- α-toxin antibody coupled immunoliposomes were generated. The effect of ISMN immunoliposomes on biofilm formation and their anti-biofilm efficacy were examined using the crystal violet method and confocal laser scanning microscopy, respectively. Relative biofilm viability at 24 h was tested using the alamarBlue assay. The biofilm formation inhibitory effect on all concentrations of ISMN immunoliposomes was stronger than that of ISMN liposomes and free ISMN (P<0.05). At concentrations of 45 and 23 mg/ml, the inhibitory effect of ISMN liposomes was stronger than that of free ISMN (P<0.05), while at 11 mg/ml, the inhibitory effect of ISMN liposomes was the same as that of ISMN (P>0.05). At 45 and 23 mg/ml, the inhibitory effect of ISMN immunoliposomes on formed biofilms was greater than that of ISMN liposomes and free ISMN (P<0.05) and the inhibitory effect of ISMN liposomes was stronger than that of free ISMN (P<0.05). At 11 mg/ml, ISMN immunoliposomes, ISMN liposomes, and ISMN had the same effect on formed biofilms (P>0.05). In conclusion, ISMN immunoliposomes nearly completely destroy biofilm structure. ISMN immunoliposomes provide a promising approach for treating infectious diseases caused by biofilms, including refractory CRS, chronic skin infection, sepsis, and osteomyelitis.
（）与顽固性慢性感染相关，尤其是在慢性鼻-鼻窦炎（CRS）中。感染和生物膜会导致术后效果较差。我们研发了负载单硝酸异山梨酯（ISMN）并与抗α毒素（抗α-毒素）抗体偶联的纳米颗粒，其可靶向生物膜，并研究了它们的抗生物膜作用。制备了抗α-毒素抗体偶联免疫脂质体。分别使用结晶紫法和共聚焦激光扫描显微镜检查了ISMN免疫脂质体对生物膜形成的影响及其抗生物膜功效。使用alamarBlue检测法测试了24小时时的相对生物膜活力。所有浓度的ISMN免疫脂质体对生物膜形成的抑制作用均强于ISMN脂质体和游离ISMN（P<0.05）。在45和23mg/ml浓度下，ISMN脂质体的抑制作用强于游离ISMN（P<0.05），而在11mg/ml时，ISMN脂质体的抑制作用与ISMN相同（P>0.05）。在45和23mg/ml时，ISMN免疫脂质体对已形成生物膜的抑制作用大于ISMN脂质体和游离ISMN（P<0.05），且ISMN脂质体的抑制作用强于游离ISMN（P<0.05）。在11mg/ml时，ISMN免疫脂质体、ISMN脂质体和ISMN对已形成生物膜的作用相同（P>0.05）。总之，ISMN免疫脂质体几乎完全破坏生物膜结构。ISMN免疫脂质体为治疗由生物膜引起的传染病提供了一种有前景的方法，包括难治性CRS、慢性皮肤感染、败血症和骨髓炎。
