Sarnat H B, Seagram G F, Trevenen C L, Rubin S Z
Am J Clin Pathol. 1985 Jun;83(6):722-5. doi: 10.1093/ajcp/83.6.722.
Acridine orange, a rapid and technically simple fluorochromic stain of nucleic acids, was applied to sections of 18 specimens of resected distal colon in infants with Hirschsprung's disease. Neurons were readily identified in the submucosal and myenteric plexuses of normal ganglionic segments because of their intense orange cytoplasmic fluorescence of ribonucleic acid (RNA) that contrasted with the pale green of the smooth muscle and connective tissue. This technic, which may be applied to both frozen and paraffin-embedded sections, is a useful adjunct in the confirmation of aganglionic segments of bowel wall.
吖啶橙是一种快速且技术上简单的核酸荧光染色剂，应用于18例患有先天性巨结肠症的婴儿切除的远端结肠标本切片。在正常神经节段的黏膜下和肌间神经丛中，神经元很容易被识别，因为它们的核糖核酸（RNA）具有强烈的橙色细胞质荧光，与平滑肌和结缔组织的淡绿色形成对比。这种技术可应用于冷冻切片和石蜡包埋切片，是确认肠壁无神经节段的有用辅助方法。
