Hanani M, Udassin R, Ariel I, Freund H R
Laboratory of Experimental Surgery, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel.
J Pediatr Surg. 1993 Jul;28(7):939-41. doi: 10.1016/0022-3468(93)90702-m.
A simple and reliable method is described for staining the nerve plexuses of the human gastrointestinal tract in whole-mounts. The staining does not require fixation and consists of a single step of 6 to 10 minutes incubation in a solution of the fluorescent dye 4-(4-diethylaminostyryl)-methylpyridinium iodide. Aganglionic bowel specimens from 6 patients with Hirschsprung's disease were stained by this method and displayed the absence of ganglion cells and an abundance of nerve fibers in the myenteric and submucosal plexuses. This staining may be suitable for the diagnosis of Hirschsprung's disease.
本文描述了一种简单可靠的方法，用于对人胃肠道神经丛进行整装染色。该染色无需固定，只需在荧光染料4-(4-二乙氨基苯乙烯基)-甲基碘化吡啶溶液中孵育6至10分钟这一步骤。用该方法对6例先天性巨结肠病患者的无神经节肠标本进行染色，结果显示肌间神经丛和黏膜下神经丛中无神经节细胞且有大量神经纤维。这种染色方法可能适用于先天性巨结肠病的诊断。
