a Department of Laboratory Animal Science, School of Basic Medical Science , Capital Medical University , Beijing , China.
Avian Pathol. 2013 Dec;42(6):541-5. doi: 10.1080/03079457.2013.845292. Epub 2013 Oct 2.
Newcastle disease, which is caused by Newcastle disease virus (NDV), is a highly contagious viral disease of poultry and other bird species. The mucosa is the first line of defence to invading pathogens, including NDV, and it has been confirmed that the mucosa can contribute to host protection. This study was conducted to evaluate the intestinal mucosal immunology in NDV infection. Forty specific-pathogen-free chickens were divided into two groups, 20 birds in each group. Group 1 was inoculated with NDV by the intravenous route. Group 2 was used as the control group and was given sterile phosphate-buffered saline by the same route. At 24, 48, 72, and 96 h post infection (h.p.i.), five chickens from each treatment were killed. Samples of the duodenum, jejunum, and ileum were collected to quantify intestinal intraepithelial lymphocytes (IEL), goblet cells and secretory IgA (sIgA) by cytochemistry and immunohistochemistry analysis. The results indicated that IEL were increased from 24 to 72 h.p.i. in the infected tissues, and were significantly higher than in the control group at 48 h.p.i. (P < 0.01). In contrast to IEL, goblet cell numbers were reduced dramatically from 24 to 96 h.p.i. in the infected birds (P < 0.01) Furthermore, the content of sIgA was significantly higher at 48 and 72 h.p.i. in the infected tissues (P < 0.01). sIgA positivity was observed in the epithelial lining of the intestinal mucosa. These data suggest that IEL, goblet cells, and sIgA were involved in the intestinal mucosal immunity against NDV infection.
新城疫是由新城疫病毒（NDV）引起的一种高度传染性的家禽和其他鸟类疾病。黏膜是抵御入侵病原体（包括 NDV）的第一道防线，现已证实黏膜有助于宿主保护。本研究旨在评估 NDV 感染中的肠道黏膜免疫学。将 40 只无特定病原体鸡分为两组，每组 20 只。第 1 组通过静脉途径接种 NDV。第 2 组作为对照组，通过相同途径给予无菌磷酸盐缓冲盐水。感染后 24、48、72 和 96 小时（h.p.i.），每组各杀死 5 只鸡。采集十二指肠、空肠和回肠样本，通过细胞化学和免疫组织化学分析定量肠上皮内淋巴细胞（IEL）、杯状细胞和分泌型 IgA（sIgA）。结果表明，感染组织中的 IEL 从 24 小时增加到 72 小时，在 48 小时时显著高于对照组（P < 0.01）。与 IEL 相反，杯状细胞数量从 24 小时到 96 小时急剧减少（P < 0.01）。此外，感染组织中 48 和 72 小时时 sIgA 的含量显著升高（P < 0.01）。sIgA 阳性在肠黏膜上皮衬里中观察到。这些数据表明，IEL、杯状细胞和 sIgA 参与了肠道黏膜对 NDV 感染的免疫反应。
