Ishida Yuuichi, Sakai Eishi, Sato Katsuo, Sugiyama Einori, Mima Kazuyuki, Taneno Akira, Shimomura Hirofumi, Cui Longzhu, Hirai Yoshikazu
Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.
Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan.
Food Saf (Tokyo). 2018 Nov 20;6(4):151-155. doi: 10.14252/foodsafetyfscj.2018003. eCollection 2018 Dec.
subsp. serovar Enteritidis (SE) is one of the major causes of food poisoning. Much effort has been made to develop a vaccine for the prevention of SE colonization and infection in poultry. However, the effect of inactivated whole-cell SE vaccines on the bacterial attachment has not been clarified. This study investigated the immune responses to a killed whole-cell SE vaccine in chickens and the effect of vaccination on the bacterial attachment of SE to cultured Vero cells. A 1 ml dose of 10-10 CFU viable SE bacterial cells was orally administered to chickens at 4 weeks or 10 months post vaccination. The number (CFU) of SE in 1 g of cecal droppings was counted on day 6 after administration. The SE CFUs were significantly lower ( < 0.05) in the vaccinated chickens, not only at 4 weeks but also at 10 months after vaccination, than in the unvaccinated control chickens. Anti-SE IgG and anti-SE IgA were detected using enzyme-linked immunosorbent assay (ELISA) in serum and intestinal and oviduct fluid samples from vaccinated chickens. Adhesion of heat-killed SE cells to Vero cells was reduced by pre-treatment of the bacteria by the vaccinated chicken-derived intestinal fluid, indicating the potential of the vaccine-induced antibody to prevent SE adhesion to epithelial cell surfaces.
肠炎沙门氏菌肠炎亚种（SE）是食物中毒的主要原因之一。人们为开发预防家禽SE定植和感染的疫苗付出了很多努力。然而，灭活全细胞SE疫苗对细菌黏附的影响尚未明确。本研究调查了鸡对灭活全细胞SE疫苗的免疫反应以及疫苗接种对SE黏附于培养的Vero细胞的影响。在接种疫苗后4周或10个月，给鸡口服1 ml剂量的10⁻¹⁰ CFU活SE细菌细胞。给药后第6天，计算1 g盲肠粪便中SE的数量（CFU）。接种疫苗的鸡在接种后4周和10个月时，其SE CFU均显著低于（<0.05）未接种疫苗的对照鸡。使用酶联免疫吸附测定（ELISA）在接种疫苗鸡的血清、肠液和输卵管液样本中检测到抗SE IgG和抗SE IgA。接种疫苗鸡的肠液预处理细菌可减少热灭活SE细胞对Vero细胞的黏附，表明疫苗诱导的抗体具有预防SE黏附于上皮细胞表面的潜力。
