Wallis R S, Alde S L, Havlir D V, Amir-Tahmasseb M H, Daniel T M, Ellner J J
Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
J Clin Invest. 1989 Jul;84(1):214-9. doi: 10.1172/JCI114143.
Pleural tuberculosis constitutes a human model of local protective immunity to mycobacterial infection as the disease is usually self-limited and recurrent pleurisy is rare. To identify potentially protective antigens of Mycobacterium tuberculosis, 37 human pleural fluid B cell clones were established using EBV and their supernatants assayed by ELISA and Western blot for antibody reactivity with M. tuberculosis sonicate and culture filtrate. One antibody identified 29,000, 31,000, and 33,000 bands in culture filtrate, and 31,000, 33,000, and 47,000 bands in sonicate; its species reactivity by ELISA was limited to M. tuberculosis. Eight antibodies identified a 31,000 band in culture filtrate and a 68,000 band in M. tuberculosis sonicate, suggesting recognition of a secreted antigen. The species crossreactivity of these eight antibodies extended to M. avium. Six antibodies identified multiple bands and had crossreactivity that included M. avium and M. kansasii. There was no reactivity with recombinant M. tuberculosis 65,000 antigen. Tuberculous pleurisy may prove useful in the identification of potentially protective mycobacterial antigens, particularly those secreted during active infection, and thus accessible to the human immune response.
胸膜结核构成了人类对分枝杆菌感染的局部保护性免疫模型，因为该疾病通常具有自限性，复发性胸膜炎罕见。为了鉴定结核分枝杆菌潜在的保护性抗原，利用EBV建立了37个人胸腔积液B细胞克隆，并通过ELISA和蛋白质印迹法检测其培养上清液与结核分枝杆菌超声破碎物和培养滤液的抗体反应性。一种抗体在培养滤液中鉴定出29000、31000和33000条带，在超声破碎物中鉴定出31000、33000和47000条带；通过ELISA检测其种属反应性仅限于结核分枝杆菌。8种抗体在培养滤液中鉴定出一条31000条带，在结核分枝杆菌超声破碎物中鉴定出一条68000条带，提示识别一种分泌抗原。这8种抗体的种属交叉反应性扩展至鸟分枝杆菌。6种抗体鉴定出多条条带，其交叉反应性包括鸟分枝杆菌和堪萨斯分枝杆菌。与重组结核分枝杆菌65000抗原无反应性。结核性胸膜炎可能有助于鉴定潜在的保护性分枝杆菌抗原，特别是那些在活跃感染期间分泌的抗原，因此人类免疫反应可以接触到这些抗原。
