a Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional , Guillermo Massieu Helguera 239, Fraccionamiento "La Escalera", Ticomán, 07320 , México DF México.
Nat Prod Res. 2013;27(24):2347-50. doi: 10.1080/14786419.2013.828289. Epub 2013 Oct 2.
Osteoarthritis is characterised by progressive loss of articular cartilage through the increase of catabolic metalloproteinases, and chondrocyte cytoskeleton disruption has also been reported. In this regard, we studied the effect of Heterotheca inuloides essential oil (HIEO) on the distribution and immunolocalisation of actin, vimentin and tubulin of chondrocytes from cultured rat articular cartilage explants in the presence of the cytoskeleton disassembly agent acrylamide. After 48 h, chondrocytes treated with acrylamide showed changes in actin immunolocalisation and shrinkage, loss of tubulin compartmentalisation and vimentin collapse and redistribution. However, the immunostaining pattern of these three proteins in acrylamide- and HIEO-treated chondrocytes simultaneously retained their typical characteristics. These results suggest that HIEO promotes protein cytoskeleton reorganisation without providing a preventive effect of acrylamide-associated disassembly. However, it is also possible that HIEO prevents vimentin disorganisation by chemical interaction with acrylamide.
骨关节炎的特征是通过增加分解代谢金属蛋白酶导致关节软骨的进行性丧失，并且已经报道了软骨细胞细胞骨架的破坏。在这方面，我们研究了 Heterotheca inuloides 精油 (HIEO) 在存在细胞骨架解聚剂丙烯酰胺的情况下对培养的大鼠关节软骨外植体中软骨细胞的肌动蛋白、波形蛋白和微管蛋白分布和免疫定位的影响。48 小时后，用丙烯酰胺处理的软骨细胞表现出肌动蛋白免疫定位和收缩的变化，微管蛋白区室化和波形蛋白崩溃和再分布的丧失。然而，丙烯酰胺和 HIEO 处理的软骨细胞中这三种蛋白质的免疫染色模式仍然保留其典型特征。这些结果表明，HIEO 促进蛋白质细胞骨架的重组，而没有提供丙烯酰胺相关解聚的预防作用。然而，也有可能 HIEO 通过与丙烯酰胺的化学相互作用来防止波形蛋白的解聚。
